Kit for detecting monohydric phenol metabolites in urine and preparation method thereof
A technique for detecting monohydric phenols and detection reagents, which is applied in the field of kits for detecting monohydric phenol metabolites in urine and its preparation field, can solve problems affecting detection results, etc., achieve obvious color development effect, short detection time, The effect of high accuracy
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Embodiment 1
[0024] A test kit for detecting monohydric phenolic metabolites in urine, comprising an ampoule filled with detection reagents, the detection reagents being chitosan composite cellulose aerogels adsorbed with color-developing mixtures. Its preparation method is:
[0025] (1) Dissolve the natural cellulose with sodium hydroxide to obtain a 4wt% cellulose solution, then cast or coat the cellulose solution into a film, and prepare a fiber with a thickness of 20 microns by coagulation regeneration and water washing Plain film;
[0026] (2) casting or coating one deck chitosan solution on the cellulose film that step (1) prepares, the solvent that dissolves chitosan is acetic acid, and the chitosan concentration that prepares is 5wt%; After coagulating, obtain a Chitosan film with a layer thickness of 20 μm;
[0027] (3) Replace the cellulose-chitosan composite membrane obtained in step (2) with absolute ethanol, remove the moisture therein, and then reverse the cellulose-chitosa...
Embodiment 2
[0034] A test kit for detecting monohydric phenolic metabolites in urine, comprising an ampoule filled with detection reagents, the detection reagents being chitosan composite cellulose aerogels adsorbed with color-developing mixtures. Its preparation method is:
[0035] (1) Dissolve the natural cellulose with sodium hydroxide and urea to obtain a 6wt% cellulose solution, then cast or coat the cellulose solution into a film, regenerate by coagulation and wash with water, and prepare a thickness of 25 microns the cellulose film;
[0036] (2) casting or coating one deck chitosan solution on the cellulose film that step (1) prepares, the solvent that dissolves chitosan is acetic acid, and the chitosan concentration that prepares is 3wt%; Obtain a Chitosan film with a layer thickness of 25 μm;
[0037] (3) Replace the cellulose-chitosan composite membrane obtained in step (2) with absolute ethanol, remove the moisture therein, and then reverse the cellulose-chitosan composite me...
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