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Antibody-regulated double-antigen-specific T cells, preparation method and application thereof

A specific, double-antigen technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, receptor/cell surface antigen/cell surface determinant, botany equipment and methods, etc., can solve Inability to identify and kill TNBC, poor efficacy of triple-negative breast cancer, manslaughter of normal cells, etc., to achieve the effect of ensuring specific recognition and killing, avoiding manslaughter injury, and avoiding accidental injury

Active Publication Date: 2018-01-09
SHENZHEN MORECELL BIOMEDICAL TECH DEV CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to overcome the above-mentioned deficiencies of the prior art, and provide a double-antigen-specific T cell and its preparation method to solve the current technology that cannot effectively identify and kill TNBC, and is likely to cause accidental killing of normal cells question
[0006] Another object of the embodiments of the present invention is to provide a pharmaceutical preparation targeting triple-negative breast cancer cells to solve the technical problems of poor curative effect and damage to normal cells in the existing biological treatment of triple-negative breast cancer

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  • Antibody-regulated double-antigen-specific T cells, preparation method and application thereof
  • Antibody-regulated double-antigen-specific T cells, preparation method and application thereof
  • Antibody-regulated double-antigen-specific T cells, preparation method and application thereof

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[0030] On the other hand, on the basis of the double-antigen-specific T cells of the embodiment of the present invention described above, the embodiment of the present invention also provides a preparation method of the double-antigen-specific T cells of the embodiment of the present invention. The preparation method of the embodiment of the present invention comprises the following steps:

[0031] T cells were simultaneously infected with the first lentivirus and the second lentivirus.

[0032] Wherein, the first lentivirus contains the above-mentioned mesothelin antigen-specific T cell receptor gene. In one embodiment, the first lentivirus is prepared according to the following method:

[0033] S11: Mix the Meso / scFv-CD8-CD3ζ gene fragment with the entry vector, so that the Meso / scFv-CD8-CD3ζ gene fragment is embedded in the entry vector to obtain the Meso / scFv-CD8-CD3ζ gene fragment Entry carrier;

[0034] S12: mixing and recombining the entry vector carrying the Meso / sc...

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Abstract

The invention provides a double-antigen specific T cell, a method for preparing the same and application of the double-antigen specific T cell. The double-antigen specific T cell comprises mesothelin antigen specific T cell receptor genes and antibody Fc (fibrillar center) fragment specific T cell receptor genes. The antibody Fc fragment specific T cell receptor genes can be mediated by HIF-1 alpha (hypoxia inducible factor-1 alpha) antibodies to specifically recognize HIF-1 alpha antigens. The method includes simultaneously infecting T cells by slow viruses with mesothelin antigen specific T cell receptor genes and slow viruses with antibody Fc fragment specific T cell receptor genes. The double-antigen specific T cell, the method and the application in an embodiment of the invention have the advantages that the double-antigen specific T cell has two specific binding domains and double-specificity for targeting to Meso (mesothelin) and being mediated by the HIF-1 alpha antibodies to specifically recognize the HIF-1 alpha antigens, accordingly, TNBC (triple negative breast cancer) cells can be assuredly specifically recognized and killed by the double-antigen specific T cell, and normal cells of organisms can be prevented from being mistakenly killed.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a double-antigen-specific T cell and a production method thereof, and a pharmaceutical preparation containing double-antigen-specific T cells targeting triple-negative breast cancer cells. Background technique [0002] T lymphocytes are the main effector cells involved in tumor cell immunotherapy, and the activation of T cells is carefully regulated by "dual signals". The first signal is the combination of a specific receptor TCR (T cell receptor) molecule on the surface of T lymphocytes and the antigen peptide presented by the major histocompatibility complex (MHC) on the surface of tumor cells, that is, the T cell’s response to the antigen. Recognition; the second signal is a signal mediated by the interaction of co-stimulatory molecules with corresponding receptors or ligands on the surface of T cells. CD28 / B7 is an important positive co-stimulatory mol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/10C12N15/867A61K39/395A61K47/46A61P35/00
CPCA61K47/46A61K2039/505C07K14/7051C07K14/70517C07K14/70521C07K14/70535C07K16/28C07K16/3015C07K2317/622C07K2319/00C12N5/0636C12N15/86C12N2740/15043
Inventor 不公告发明人
Owner SHENZHEN MORECELL BIOMEDICAL TECH DEV CO LTD
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