Antigen for detecting human papillomavirus antibody and related immunoassay kit

一种抗人乳头瘤、免疫检测的技术，应用在检测抗人乳头瘤病毒抗体的抗原及相关免疫检测试剂盒领域，能够解决高假阳性率、无法分辨一过性感染等问题，达到减轻痛苦、适于大规模推广应用、防止癌症发生的效果

Active Publication Date: 2017-05-10
ATTOGEN BIOMEDICAL SUZHOU INC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the defect of HPV DNA detection is that it cannot distinguish between transient infection and infection that can cause cervical lesions, resulting in a high false positive rate

Method used

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  • Antigen for detecting human papillomavirus antibody and related immunoassay kit
  • Antigen for detecting human papillomavirus antibody and related immunoassay kit
  • Antigen for detecting human papillomavirus antibody and related immunoassay kit

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Embodiment Construction

[0019] In order to understand the technical content of the present invention more clearly, the following specific examples are enumerated in detail. However, the specific examples are for illustration only, not limitation of the present invention.

[0020] 1. Materials and methods

[0021] 1. Reagents and medicines

[0022] Horseradish peroxidase-labeled goat anti-human (Goat Anti-Human IgG, FC) was purchased from Priece Product#31413.

[0023] Sodium chloride, potassium chloride, disodium hydrogen phosphate, and potassium dihydrogen phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd. Tween-20 was purchased from AMRESCO. Fetal bovine serum was purchased from Beijing Yuanheng Jinma Biotechnology Development Co., Ltd.

[0024] 2. Instruments and consumables

[0025]Electronic balance (Mettler-Toledo Instruments Shanghai Co., Ltd.); 85-1 constant temperature magnetic stirrer (Jiangsu Jintan Jerry Electric Co., Ltd.); MULTISKAN MK3 microplate reader (Thermo Com...

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Abstract

The invention relates to a human papillomavirus (HPV) oncogene protein which is used for detecting an HPV antibody as an antigen. The sequence of the antigen is shown in SEQ ID NO: 4. The invention further provides an immunoassay kit containing the antigen and an application of the antigen and the kit. The antigen for detecting the HPV antibody, provided by the invention, can be used for detecting the HPV antibody in blood or saliva in a high-sensitivity, high-specificity and high-stability manner, so that a foundation for accurately diagnosing the cervical intraepithelial neoplasias and cervical cancer, transformed from HPV oncogene, of patients by doctors can be brought, and then, treatment is adopted in time so as to prevent cancer occurrence or cancer diffusion and relieve patient's sufferings in time; and the immunoassay kit prepared from the antigen can be used for rapidly, simply and accurately detecting the HPV antibody in the blood or saliva, thereby being suitable for being popularized and applied on a large scale.

Description

[0001] This application is a divisional application of an invention application with an application date of February 22, 2013, an application number of 201310056516.3, and an invention title of "antigen for detecting anti-human papillomavirus antibodies and related immunoassay kits". technical field [0002] The present invention relates to the technical field of antibody detection, in particular to the technical field of anti-human papillomavirus (Human Papillomavirus, HPV) antibody detection, in particular to an antigen and a related immunoassay kit for detecting anti-human papillomavirus antibodies, especially available High-risk human papillomavirus (HPV) subtypes HPV16 and HPV18 prevalent in China. Background technique [0003] Cervical cancer is the second most common female malignancy, and human papillomavirus (HPV) is necessary for its development. Although HPV infection is very common in the population, for example, the infection rate of women in areas with a high i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/025G01N33/574G01N33/569
CPCC07K14/005C12N2710/20022G01N33/543G01N33/56983G01N33/57442G01N33/68G01N2333/025C07K16/084C12N2710/20031
Inventor 常小迦张杰成许虹
Owner ATTOGEN BIOMEDICAL SUZHOU INC
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