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Method for preparing two chemical reference substances of Rhoifolin and naringin simultaneously

A technology for anacardin and naringin, which is applied in the field of simultaneously preparing two chemical reference substances of anacardin and naringin, can solve the problems of large amount of organic solvent, complicated process and high cost, and achieves low cost and simple process. , a rich source of effects

Inactive Publication Date: 2017-05-10
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Solvent method extracts rhodium from orange leaves, separates and removes chlorophyll and naringin through liquid-liquid extraction, and then obtains rhodium with high purity through crystallization and recrystallization, but a large amount of naringin needs to be removed, and the process is complicated , the amount of organic solvent is large, the yield is low, and the cost is high

Method used

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  • Method for preparing two chemical reference substances of Rhoifolin and naringin simultaneously
  • Method for preparing two chemical reference substances of Rhoifolin and naringin simultaneously

Examples

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Effect test

Embodiment 1

[0030] (1) Ethanol extraction: Crush the red leaves of tangerines, pass through a 40-mesh sieve, weigh 1kg of powder, and evenly load it into a glass chromatography column with 95% wet method, use 95% ethanol as the eluent, and the eluent speed is 10mL / min, collect 2L of eluate.

[0031] (2) Extraction of n-butanol-petroleum ether mixture: the collected eluate was concentrated by rotary evaporation under reduced pressure to recover the solvent, and about 125mL of the ethanol extract of Huajuhongye was obtained. Wash the residue with water, put it into a separatory funnel, 200mL in total, extract 3 times with 200mL n-butanol-petroleum ether (V / V, 1:9) mixture, remove the leaf pigment solution in the upper layer, and obtain the precipitate and solution containing the target components in the lower layer.

[0032] (3) Cooling and precipitation: Transfer the lower layer obtained above to a centrifuge tube, adjust the pH to 4 with hydrochloric acid, put it in a refrigerator at 4°C...

Embodiment 2

[0036] (1) Ethanol extraction: Crush the red leaves of oranges, pass through a 40-mesh sieve, weigh 1kg of powder, and evenly load it into a glass chromatography column with 95% moisture, use 95% ethanol as the eluent, and the elution rate is 10mL / min , collect 3 L of eluate.

[0037] (2) Extraction of n-butanol-petroleum ether mixture: the collected eluate was concentrated by rotary evaporation under reduced pressure to recover the solvent, and about 150mL of the ethanol extract of Huajuhongye was obtained. Wash the residue with water, put it into a separatory funnel, 200mL in total, extract 4 times with 200mL n-butanol-petroleum ether (V / V, 1:9) mixture, remove the leaf pigment solution in the upper layer, and obtain the precipitate and solution containing the target components in the lower layer.

[0038] (3) Cooling and precipitation: transfer the lower layer obtained above to a centrifuge tube, adjust the pH to 4 with hydrochloric acid, put it in a refrigerator at 4°C ove...

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Abstract

The invention discloses a method for preparing two chemical reference substances of Rhoifolin and naringin simultaneously. The method comprises the following steps of simultaneously extracting Rhoifolin and naringin in exocarpium leaves with an ethanol solution, after vacuum concentration of an extracting solution, extracting with an n-butyl alcohol-petroleum ether mixed solution to remove chromophyll, putting the lower part in a refrigerator for standing, washing with cold water and centrifuging, removing impurities such as water-soluble polysaccharide and proteins, dissolving a crude product with methyl alcohol, then using a high performance liquid preparative chromatograph for separation, and thus obtaining the two reference substances of Rhoifolin and naringin with the purity of more than 98%. According to the method, the reference substances of Rhoifolin and naringin can be prepared simultaneously, the processing step is simple, the purity is high, the cost is low, and the preparation quantity is large.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to a method for simultaneously preparing two kinds of chemical reference substances rhozoin and naringin. Background technique [0002] Rhoifolin is a flavonoid compound, derived from the dried leaves and fruits of Rutaceae and Rutaceae. Pharmacological effects: anti-oxidation, anti-tumor, anti-hypertension, xanthine oxidase inhibitor, because it is Xanthine oxidase inhibitors can effectively inhibit the generation of uric acid in the body, so they are special ingredients for the development of new drugs for the treatment of gout. [0003] Naringin (Naringin) is a dihydroflavonoid compound with a bitter taste. It naturally exists in the peel and pulp of grapefruit, tangerine, and orange in the Rutaceae plant. One of the main active ingredients of traditional Chinese medicine. Naringin has various biological activities, such as: anti-oxidation, anti-cancer, anti-inflam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H17/07C07H1/08
Inventor 韩寒冰尹爱国杨培苑李颖刘杰凤刘成有
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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