A low-salt fermentation method of watercress and pepper base based on microbial enhancement and pouring in the original pond
A pool pouring and microbial technology, which is applied in the field of low-salt fermentation of watercress and pepper blanks, to achieve the effect of reducing salt concentration and avoiding unstable fermentation
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Embodiment 1
[0022] A low-salt fermentation method for watercress chili base based on microbial enhancement and pouring in the original pond, the steps are:
[0023] (1) Pretreatment of chili billet
[0024] Take the red pepper freshly harvested in the season, remove the pepper, wash, drain, crush, add 13-18% w / w edible salt, mix evenly, put it in a storage pool with a false bottom, and salt for 3-10 days;
[0025] (2) Preparation of compound microbial fermentation broth
[0026] Xanthomonas sp. CICC 10257 was cultured in the Xanthomonas medium at 30°C for 48-72 hours, so that the total number of viable bacteria reached 10 9 More than CFU / g;
[0027] Saccharomyces cerevisiae CICC 31016 was cultured in Saccharomyces cerevisiae medium at 30°C for 16-24 hours, so that the total number of viable bacteria reached 10 8 More than CFU / g;
[0028] Bacillus subtilis (CICC 20520) and Lactobacillus plantarum (Lactobacillus plantarum BNCC194165) were cultured in the combined medium at 37°C for 48-7...
Embodiment 2
[0037]Annex 2, Detection methods for volatile components such as alcohols, aldehydes, acids, esters, etc.
[0038] Solid-phase microextraction device, Supelco Company of the United States; QP2010plus gas chromatography-mass spectrometer, Shimadzu Corporation of Japan; constant temperature circulation tank, Shanghai Yiheng Scientific Instrument Co., Ltd.; 90mm mortar, Shengxing Chemical Porcelain Factory, Kaiping, Tangshan City.
[0039] 1 Sample pretreatment
[0040] Take about 20 g of watercress from a homogeneously mixed sample, grind it in a mortar until it becomes a uniform paste, seal the bag and store it in a freezer at -20°C. All samples should be assayed within 3 days of retrieval.
[0041] 2 HS-SPME method extraction
[0042] Weigh 5 g of the ground sample, put it into a 15 mL sealed headspace bottle, and place it in a water bath at 55 °C for 20 min to equilibrate. Then the aged 75 μm CAR / PDMS extraction head was inserted into the headspace bottle for adsorption fo...
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