Planting method and applications for increasing yield of active ingredients in taxus media

A technology of taxus mandiensis and active ingredients, which is applied in the directions of botanical equipment and methods, fertilization methods, applications, etc., can solve the problem of harsh temperature range, no increase in the yield of taxus chinensis, and no increase in the yield per unit of taxol of taxus chinensis plants and other problems, to achieve the effect of taking into account social benefits, alleviating the shortage of raw materials, and solving local employment problems

Active Publication Date: 2017-05-10
CHONGQING BEISHENG PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, no matter which method can increase the unit yield of taxus in Taxus plants, although the three-dimensional cultivation of the former has improved the output benefit per unit of land, the output of Taxus per unit of land has not increased, and the temperature selected by the latter The range of 8-12°C is very harsh and can only be implemented in some areas in the north

Method used

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  • Planting method and applications for increasing yield of active ingredients in taxus media

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preparation example Construction

[0026] 1. Preparation of composite microbial agent

[0027] (1) The spores of the AM fungus Tonoglossomyces were treated with 30% H 2 o 2 Disinfect for 10 minutes, then rinse with sterile water for 3 times, enrich and cultivate the spores with white clover as the carrier, the culture medium is a mixture of sterilized soil and sand, the mass ratio of soil and sand is 2:1, after 2 months of cultivation, remove Aerial parts, made into a mixture containing spores, hyphae and infected heel segments as AM fungal inoculants;

[0028] (2) Activate Bacillus subtilis, use an inoculation loop to pick a small amount of bacteria and inoculate it into 5 mL of LB medium. The specific components of LB medium are 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, 1000 mL of distilled water, and pH 7.2 ~7.4, sterilized at 121°C for 30 minutes. Cultivate in a 20mL culture flask overnight at 37°C with shaking at 200r / min, and use it as a seed solution, transfer it to a 500mL Erlen...

Embodiment 1

[0034] 1. 10 days before transplanting every year, that is, from late September to early October every year, choose an altitude of about 800-1800 meters, a perennial temperature between -5 and 35°C, humid air, and rainfall between 1000-2000mm The soil is loose and permeable, the soil is fine, no large particles, the soil slope is less than 15°, and the thickness of the soil layer is more than 50 cm. Firstly, the soil is plowed at a depth of 40-50 cm, and the organic fertilizer produced by our company is mixed with the soil evenly at an amount of 20 kg / mu, and the land is leveled for use.

[0035] 2. During the period from mid-October to late October every year, the Mandia yew saplings that have been raised for one year in the nursery are raised from the whole plant, paying attention to the integrity of the roots of the seedlings. Soak the roots of the seedlings in an aqueous solution of ABT rooting powder with a rooting agent mass fraction of 10% for 3 to 5 seconds, and then t...

Embodiment 2

[0039] 1. 10 days before transplanting every year, that is, from late September to early October every year, choose an altitude of about 800-1800 meters, a perennial temperature between -5 and 35°C, humid air, and rainfall between 1000-2000mm The soil is loose and permeable, the soil is fine, no large particles, the soil slope is less than 15°, and the thickness of the soil layer is more than 50 cm. Firstly, the soil is plowed at a depth of 40-50 cm, and the organic fertilizer produced by our company is mixed with the soil evenly at an amount of 10 kg / mu, and the land is leveled for use.

[0040] 2. During the period from mid-October to late October every year, the Mandia yew saplings that have been raised for one year in the nursery are raised from the whole plant, paying attention to the integrity of the roots of the seedlings. Soak the roots of the seedlings in an aqueous solution of 5% naphthaleneacetic acid for 3 to 5 seconds, and then transplant them into the land prepar...

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Abstract

The invention relates to a planting method and applications for increasing the yield of active ingredients in taxus media. The planting method for increasing the yield of active ingredients in taxus media comprises the following steps: land leveling, seedling planting, fertilizer application management and the like. The roots of the taxus media planted by utilizing the method are developed, the trunk and branches collected from the taxus media grown for two years are about 50 to 60cm long, the weight ratio of branches to roots is (1:1) to (1:1.5), and the content of active ingredients in the root part of the taxus media is three to five times that of branches and leaves. The yield of the active ingredients in the taxus media planted by utilizing the method is two to three times that from the traditional trimmed branches, the problem of short paclitaxel bulk pharmaceutical chemical resources can be effectively relieved, a great amount of taxus media seedlings can be planted every year, and the local employment problem can be obviously solved, so that social benefits can be considered while economic benefits are guaranteed.

Description

technical field [0001] The invention belongs to the field of agricultural planting, and in particular relates to a planting method and application for increasing the yield of active ingredients in Taxus chinensis. Background technique [0002] Mandia yew (Taxus X media), native to the United States and Canada, is a natural hybrid variety. Its female parent is Northeast yew (T.cuspidata), and its male parent is European yew (T.baccata). It has been grown and developed in the United States and Canada for nearly 100 years. It is an evergreen shrub with huge biomass and short growth time. The main root is not obvious, the lateral roots are developed, the branches and leaves are lush, the germination is strong, and it is resistant to low cold, and can withstand the low temperature of -25°C. Its tree shape is peculiar and has high ornamental value. [0003] "Paclitaxel" contained in yew has excellent anti-cancer effect. It has been proved by clinical experiments that paclitaxel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G17/00A01C21/00C05F3/00
CPCA01C21/005A01G17/005C05F3/00C05F11/00C05F11/08Y02A40/20
Inventor 潘先文余海鹏潘敬坤
Owner CHONGQING BEISHENG PHARMA TECH CO LTD
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