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Screening method of anti myocardial ischemia active components of flower of Polygonum orientale

An anti-myocardial ischemia, active ingredient technology, applied in material separation, analysis materials, measuring devices, etc., can solve the problem of unclear pharmacodynamic material basis, and achieve the effect of eliminating the interference of a large number of non-active components

Active Publication Date: 2017-04-26
GUIZHOU MEDICAL UNIV
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Problems solved by technology

Existing studies have found that the n-butanol extraction part of "Wortia radix" after being boiled in water has a more obvious anti-myocardial ischemia effect than the whole plant of "Wortia chinensis", which is the main medicinal effective part of W. The substance basis of the drug effect in the body is not yet clear, and further research and screening are needed to lay the foundation for its innovative drug research and in-depth development and utilization

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  • Screening method of anti myocardial ischemia active components of flower of Polygonum orientale

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Embodiment Construction

[0025] The specific embodiments of the present invention will be further described below in conjunction with the accompanying drawings. It should be noted here that the descriptions of these embodiments are used to help understand the present invention, but are not intended to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below may be combined with each other as long as they do not constitute a conflict with each other.

[0026] 1. Materials

[0027] 1.1 Reagent

[0028] DMEM high glucose medium (Gibco Company, USA), fetal bovine serum (Biochrom Company, GER), trypsin (Solarbio Company), phosphate buffered saline PBS (A) (PH 7.4, Boster Company, Wuhan, China), phosphate buffered saline PBS (B) (PH4, prepared by mixing PBS (A) with 1mol / L hydrochloric acid). Protocatechuic acid, catechin, kaempferin-3-O-β-D-glucoside, kaempferi-3-O-α-L-rhamnoside reference substance (self-made, purity ≥...

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Abstract

The invention discloses a screening method of anti myocardial ischemia active components of flower of Polygonum orientale, myocardial cells are used for selective combination with the active components of extract of the flower of Polygonum orientale, after other uncombined ingredients are washed away, cellular targets are inactivated, the combined active components are released from the myocardial cells, and then the UHPLC-Q-TOF / MS technology is used for analyzing and identifying. The method has the advantages that by adopting of the specific H9c2 myocardial cells, the active components of the extract of the flower of Polygonum orientale can be specifically and selectively combined with the specific H9c2 myocardial cells, the traditional Chinese medicine action multi-target screening of the active components can be met, by use of an UHPLC-Q-TOF / MS system, a reliable method of qualitative analysis of biological samples can be established, medicinal components in a myocardial cell extraction solution can be comprehensively reflected, and reference is provided for rapid determination of pharmacodynamic material basis of the flower of Polygonum orientale and innovative drug research.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to a screening method for anti-myocardial ischemia active ingredients of the sage flower. Background technique [0002] Biological activity screening / chemical composition online analysis technology is a comprehensive technology formed by the intersection of life science, chromatography and mass spectrometry separation and identification technology. Active biological macromolecules, active cell membranes, and even active cells that play important physiological roles in life activities such as ion channels and nerve mediators are used as targets to establish a biological activity screening / chemical composition online analysis method based on the effective substances of traditional Chinese medicine. Cell extraction can use the selectivity of cells to screen the components that bind to cells. There is a significant correlation between the components that bind to cells and the effect compone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
CPCG01N30/06G01N2030/062
Inventor 郑林李勇军黄勇王永林廖尚高谢玉敏李月婷巩仔鹏孙佳杨畅陆苑刘亭陈思颖
Owner GUIZHOU MEDICAL UNIV
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