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Supermolecular kit and method for quick detection of diquat

A supramolecular and kit technology, which is applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, can solve the problems of high time and cost, and reduce analysis time and cost. Effect

Inactive Publication Date: 2017-04-26
CHINA AGRI UNIV
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Problems solved by technology

However, the instrumental analysis method requires the use of expensive instruments for identification and analysis after the samples are collected back to the laboratory, which is time-consuming and expensive, especially for ordinary people, who do not have the conditions to use complex and sophisticated instruments

Method used

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  • Supermolecular kit and method for quick detection of diquat
  • Supermolecular kit and method for quick detection of diquat

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Embodiment Construction

[0015] The present invention provides a supramolecular kit and method for rapid identification and detection of diquat, and the present invention will be further described below in combination with specific embodiments.

[0016] 1. Reagents

[0017] Saffron T (ST), methylene blue (MB) and hematoxylin (HT) were purchased from Aladdin; carboxymethyl-β-cyclodextrin was purchased from Shandong Binzhou Zhiyuan Biotechnology Co., Ltd.; Diquat standard samples , purchased from the Environmental Protection Research and Monitoring Institute of the Ministry of Agriculture, and diluted with ultra-pure water to a working solution of the required concentration.

[0018] 2. Preparation of supramolecular kit

[0019] Put 0.0088g ST in a 25mL volumetric flask to obtain a 0.001mol / L ST solution, place 0.0093g MB in a 25mL volumetric flask to obtain a 0.001mol / L MB solution, and place 0.0850g CM-β-CD in a 50mL volumetric flask to obtain a 0.001mol / L of CM-β-CD solution. Take 125 μL of ST so...

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Abstract

Disclosed are a supermolecular kit and a method for quick detection of diquat. The supermolecular kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is a solution prepared from safranine T and carboxymethyl-beta-cyclodextrin, and the reagent 2 is a solution prepared from methylene blue and carboxymethyl-beta-cyclodextrin. The supermolecular kit is capable of detecting the diquat qualitatively through color matching by naked eyes and solves the problems about time and cost.

Description

technical field [0001] The invention generally relates to the field of pesticide detection, in particular to a supramolecular kit and method for detecting diquat. Background technique [0002] According to the announcements of the Ministry of Agriculture, the Ministry of Industry and Information Technology, and the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, the registration and production license of paraquat aqueous agents will be revoked and production will be suspended from July 1, 2014; Domestic sales and use. Diquat is the third largest herbicide in the world after glyphosate and paraquat. Therefore, the use of diquat as a substitute for paraquat in my country will show an increasing trend. However, because the price of diquat will be much higher than that of paraquat, some manufacturers will pursue profits and mix paraquat into the formulation of diquat, so it is necessary to conduct random inspections o...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 刘东晖刘畅毕嘉玮王鹏周志强
Owner CHINA AGRI UNIV
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