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Squid ink oligopeptide with lipid metabolism regulation function

A technology of lipid metabolism and squid ink, applied in the field of squid ink oligopeptide extract that can regulate lipid metabolism, can solve the problems of enzymatic hydrolysis efficiency to be improved, and achieve the effect of controllable product quality, small molecular weight and low cost

Inactive Publication Date: 2017-04-26
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the second step of enzymatic hydrolysis, a single enzyme is still used for hydrolysis, and there is still room for improvement in the adequacy of enzymatic hydrolysis, and no enzyme activator is added to the protease, so the enzymatic hydrolysis efficiency needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A squid ink oligopeptide extract capable of regulating lipid metabolism, the specific preparation steps are:

[0020] 1) Pretreatment: cut the ink sac or stimulate the squid to spray ink to collect squid ink;

[0021] 2) Enzymolysis: Add pepsin to squid ink, the amount added is 0.2~0.7% of the substrate mass, the enzymolysis conditions are: 45~60°C, pH 1.0~2.5, and the enzymolysis time is 0.4~0.8h. Then add the compound enzyme preparation, the compound enzyme preparation is added according to the mass ratio of the substrate at 1:100~300, the ingredients and parts by weight are: 10~15 parts of trypsin, 5~8 parts of papain, 0.003~0.007 parts of magnesium chloride, 0.001~0.005 part of calcium chloride, 0.0002~0.0005 part of 3-methoxy-4-hydroxybenzaldehyde and 0.004~0.09 part of phenylethanol glycoside. The enzymolysis temperature is 40~55℃, the pH is 6.5~7.8, and the enzymolysis time is 0.8~1.7h. No strong acid or alkali is used, the enzymolysis conditions are mild, ener...

Embodiment 2

[0026]A squid ink oligopeptide extract capable of regulating lipid metabolism, the most preferred preparation steps are:

[0027] 1) Pretreatment: cut the ink sac or stimulate the squid to spray ink to collect squid ink;

[0028] 2) Enzymolysis: Pepsin was added to the squid ink, the amount added was 0.5% of the substrate mass, the enzymolysis conditions were: 50°C, pH 1.5, and the enzymolysis time was 0.6h. Then add the compound enzyme preparation, the compound enzyme preparation is added at a mass ratio of 1:200 to the substrate, and the composition and its most preferred parts by weight are: 13 parts of trypsin, 7 parts of papain, 0.006 part of magnesium chloride, 0.005 part of calcium chloride, 0.0002 parts of 3-methoxy-4-hydroxybenzaldehyde and 0.03 parts of phenylethanol glycosides. The enzymolysis temperature is 50°C, the pH is 7.2, and the enzymolysis time is 1.5h. The use of 3-methoxy-4-hydroxybenzaldehyde and phenylethanol glycoside can avoid the use of strong acid...

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Abstract

The invention provides a squid ink oligopeptide extract with lipid metabolism regulation function. The preparation steps include: 1) pretreatment; 2) enzymolysis; 3) ultrafiltration; 4) purification; and 5) drying. The invention has the beneficial effects that: the enzymolysis conditions are mild, energy can be saved, and the ink oligopeptide activity cannot be destroyed; enzymolysis has the characteristics of high efficiency and specificity, also the unique formula of a composite enzymolysis agent can greatly shorten the preparation time, and does not cause protein loss; enzyme hydrolysis is carried out twice, enzymolysis is complete, the obtained molecular weight is small, and the squid ink oligopeptide extract can be easily absorbed by the human body; the use of a centrifugal ultrafiltration tube does not generate a concentration gradient, the separation speed is fast, and protein loss cannot be caused, the desalting effect is good, and the ink oligopeptide extraction rate is high; and ultrafiltration is carried out again for further purification of ink oligopeptide, and the obtained ink oligopeptide has high purity. According to the invention, the operation steps are simple, the production technology is advanced, the product quality is controllable, the cost is low, and the economic benefits are high.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction, in particular to a squid ink oligopeptide extract capable of regulating lipid metabolism. Background technique [0002] Lipid metabolism refers to the synthesis and decomposition process of lipids in organisms. Fat is digested mainly in the upper small intestine through the action of various enzymes and bile salts, where it is hydrolyzed into glycerol, fatty acids, etc. There are two situations for the absorption of lipids: triglycerides composed of medium-chain and short-chain fatty acids can be absorbed after emulsification, and the absorbed triglycerides are hydrolyzed into fatty acids and glycerol in intestinal mucosal cells, and finally enter the blood through the portal vein. Triglycerides composed of long-chain fatty acids are decomposed into long-chain fatty acids and monoglycerides in the intestinal tract. After reabsorption, triglycerides are re-synthesized in intestinal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 陈荫
Owner ZHEJIANG OCEAN UNIV
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