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Method for preparing feed-grade fructo-oligosaccharide by taking molasses as raw material

A technology of fructooligosaccharides and feed grade, which is applied in the field of preparing feed grade fructooligosaccharides, can solve the problems of application limitation of fructooligosaccharides, restricting industrial development, long reaction time, etc., and achieves good appearance, improved utilization rate, and reaction time. short effect

Pending Publication Date: 2021-02-09
中粮崇左糖业有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The Chinese patent application number 200610123934.X discloses "a method for preparing fructooligosaccharides from molasses". In this scheme, only fructosyltransferase is used to hydrolyze molasses, and the reaction time is long and pretreatment with concentrated sulfuric acid is required. The application of fructooligosaccharides in the industry is limited
The Chinese patent with the application number 201310677505.7 discloses "a functional concentrated fructooligosaccharide feed and its preparation method". In this patent, functional oligosaccharides are directly produced through microbial transformation, and the cultivation and activation of the strain usually takes 36-72 hours. The secondary seed liquid of Aspergillus niger also needs to be fermented for at least 120 hours, which takes too long and restricts the development of the industry

Method used

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Embodiment 1

[0035] The present embodiment provides a kind of method using molasses as raw material to prepare feed grade fructo-oligosaccharides, the method may further comprise the steps:

[0036] (1) Preparation of molasses aqueous solution: take a certain amount of molasses to prepare an aqueous solution, wherein the mass ratio of molasses to water is 2:1, mix the molasses with water and stir until the molasses is completely dissolved in water to obtain the molasses aqueous solution for subsequent use;

[0037] (2) Pretreatment of molasses aqueous solution: adjust the temperature of molasses aqueous solution to be 40° C., and the pH to be 5.0;

[0038] (3) Enzymolysis: add enzymolysis solution to the molasses aqueous solution pretreated in step (2), stir evenly, and react for 9 hours under the assistance of microwaves. The microwave power is 950W, and the frequency is 700MHz. After the above treatment, Enzymolysis solution, for use; the enzymolysis solution includes pectinase and fruct...

Embodiment 2

[0044] The present embodiment provides a kind of method using molasses as raw material to prepare feed grade fructo-oligosaccharides, the method may further comprise the steps:

[0045] (1) Preparation of molasses aqueous solution: take a certain amount of molasses to prepare an aqueous solution, wherein the mass ratio of molasses to water is 3:1, mix the molasses with water and stir until the molasses is completely dissolved in water to obtain the molasses aqueous solution for subsequent use;

[0046] (2) Pretreatment of molasses aqueous solution: adjust the temperature of molasses aqueous solution to be 50° C., and the pH to be 5.5;

[0047] (3) Enzymolysis: add enzymolysis solution to the molasses aqueous solution pretreated in step (2), stir evenly, and react for 10 hours under the assistance of microwaves. The microwave power is 850W, and the frequency is 750MHz. After the above treatment, Enzymolysis solution, for use; the enzymolysis solution includes pectinase and fruc...

Embodiment 3

[0053] The present embodiment provides a kind of method using molasses as raw material to prepare feed grade fructo-oligosaccharides, the method may further comprise the steps:

[0054] (1) Preparation of molasses aqueous solution: take a certain amount of molasses to prepare an aqueous solution, wherein the mass ratio of molasses to water is 4:1, mix the molasses with water and stir until the molasses is completely dissolved in water to obtain the molasses aqueous solution for subsequent use;

[0055] (2) Pretreatment of molasses aqueous solution: adjust the temperature of molasses aqueous solution to be 60° C., and the pH to be 5.5;

[0056] (3) Enzymolysis: add enzymolysis solution to the molasses aqueous solution pretreated in step (2), stir evenly, and react for 11 hours under the assistance of microwave. The microwave power is 1000W, and the frequency is 800MHz. After the above treatment, Enzymolysis solution, for use; the enzymolysis solution includes pectinase and fruc...

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Abstract

The invention relates to the technical field of biological processing, in particular to a method for preparing feed-grade fructo-oligosaccharide by taking molasses as a raw material. The method disclosed by the invention comprises the following steps: (1) preparing a molasses aqueous solution; (2) pretreating the molasses aqueous solution; (3) carrying out enzymolysis; (4) carrying out enzyme deactivation and (5) drying. According to the invention, the processing by-product molasses in the sugar industry is used for replacing cane sugar to prepare the feed-grade fructo-oligosaccharide, the rawmaterial is replaced, the utilization rate of the sugar processing by-product is improved, the additional value of the molasses is improved, the production cost of the fructo-oligosaccharide is reduced, the processing technology is simple, the processing time is short, and the purity of the obtained fructo-oligosaccharide is high.

Description

【Technical field】 [0001] The technical field of bioprocessing of the present invention particularly relates to a method for preparing feed grade fructo-oligosaccharides from molasses. 【Background technique】 [0002] As a by-product of the sugar industry, molasses is a dark brown, viscous, semi-flowing liquid, which can produce 3.5 million tons of waste molasses per year in the process of sugar processing alone. The content of soluble carbohydrates in molasses is relatively high, and the sugar contained is almost all sucrose, about 40-50%, and it also contains 8-10% minerals and a small amount of protein. With the rapid development of "healthy breeding" in China, the research and development and production of "antibiotic-free feed" will be the general trend, and fructooligosaccharides, because of their excellent health care functions and special physiological activities, such as promoting the growth of beneficial bacteria in the digestive tract, The effects of adsorbing inte...

Claims

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Application Information

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IPC IPC(8): C12P19/18C12P19/14A23K20/163A23K50/75A23K50/30A23K50/60
CPCC12P19/18C12P19/14A23K20/163A23K50/75A23K50/30A23K50/60
Inventor 徐光辉张涛金渭武潘莹孙铁虎梁幼飞杨钊张菡谢凌波沈雪梅
Owner 中粮崇左糖业有限公司
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