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Preparation method and application of human umbilical cord mesenchymal stem cells

A technology of mesenchymal stem cells and human umbilical cord, applied in the field of preparation of human umbilical cord mesenchymal stem cells, can solve the problems of undiscovered treatment plan, poor effect, limited effect, etc., and achieve a flat shape, reduced cleavage phase, and abundant cytoplasm Effect

Inactive Publication Date: 2017-04-26
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current measures are limited and ineffective, and new treatment options remain to be discovered

Method used

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  • Preparation method and application of human umbilical cord mesenchymal stem cells
  • Preparation method and application of human umbilical cord mesenchymal stem cells
  • Preparation method and application of human umbilical cord mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation method suitable for human umbilical cord mesenchymal stem cells

[0021] 1.1 Materials The umbilical cords of full-term caesarean section fetuses were obtained from our hospital (obtained in accordance with the hospital’s regulations and with the consent of the family members); type II collagenase (2000U / rag), DMEM-LG medium, and HEPES were purchased from Gibco; Percoll cell separation solution was purchased from Pharmacia Company; fetal bovine serum (FBS) is the product of Hangzhou Sijiqing Company; CD14, CD29, CD34, CD44, CD45 monoclonal antibody is the product of BD Company; DMSO is the product of Sigma Company, CO 2 The incubator is a product of Heraeus Company in the United States; the inverted phase-contrast microscope is a product of Shanghai Haiou Company; the 96-well culture plate is a product of Bilong Company in China.

[0022] 1.2 Isolation and in vitro culture of huMSCs: Take the umbilical cord of a full-term caesarean section fetus, c...

Embodiment 2

[0032] Example 2 Suitable for the use of human umbilical cord mesenchymal stem cells

[0033] In recent years, human umbilical cord mesenchymal stem cell therapy has been a frontier target in regenerative medicine for regeneration and the body's repair of damaged tissue. It is a promising therapeutic technique in which transplanted stem cells generate tissue that covers damaged tissue with the ability to repair and regenerate the original tissue. The following is about the brain injury (strike injury), after the human umbilical cord stem cells prepared in Example 1 were given and transplanted, the results seen are as follows.

[0034] 1. Intravenous injection of human umbilical cord mesenchymal stem cells improves functional recovery after brain injury

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Abstract

The invention discloses a preparation method and an application of human umbilical cord mesenchymal stem cells. The preparation method includes the steps of: performing asepsis treatment to umbilical cord of a full-term caesarean delivered healthy fetus, mechanically cutting the umbilical cord, digesting the umbilical cord through collagenase and performing separation cultivation. In about the third generation, cell shape becomes flat and broad and the cells grow well. The method can culture the cells till the 15th generation, wherein cell division phase is reduced and cytoplasm is loose, there are vacuoles in the cells, and cell aging and proliferation are significantly slowed, so that the third-generation cells have high activity and can be applied in subsequent researching and treatment. The human umbilical cord mesenchymal stem cells are used in therapy of cerebral injury, and improve function recovery after the cerebral injury.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application suitable for human umbilical cord mesenchymal stem cells. Background technique [0002] Human mesenchymal stem cells (hUMSCs) are a pluripotent cell population that can be easily isolated from some adult types of tissues such as bone marrow, adipose tissue, and medical waste such as umbilical cord and placenta. It has been reported that MSCs isolated from human umbilical cord have better cell content and proliferation ability than bone marrow MSCs, lower immunogenicity than bone marrow MSCs, and have the advantages of convenient material extraction and no ethical controversy. s concern. These have the potential to differentiate into a variety of cells (ie, osteoblasts, adipocytes, chondrocytes, tenocytes, and muscle cells), and have the prospect of being used in stem cell therapy. It is a cutting-edge target for the body to repair da...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K35/28A61P25/00
CPCA61K35/28C12N5/0668C12N2509/00
Inventor 王廷华
Owner KUNMING MEDICAL UNIVERSITY
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