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Phagocytosis capable of degrading penicillin, cell fractions and compositions thereof

A technology of phagocytosis and penicillin, applied in the field of phagocytosis, cell fractions and their compositions, can solve the problems of secondary pollution, high energy consumption, unpleasant exhaust gas, etc., and achieve the effect of energy saving and cost saving

Active Publication Date: 2019-08-13
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing methods for harmless treatment of penicillin mycelia mainly include thermal steaming and acid-base degradation. These methods either consume a lot of energy, or cause secondary pollution, and produce unpleasant waste gas

Method used

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  • Phagocytosis capable of degrading penicillin, cell fractions and compositions thereof
  • Phagocytosis capable of degrading penicillin, cell fractions and compositions thereof
  • Phagocytosis capable of degrading penicillin, cell fractions and compositions thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening, identification, degradation ability and sequence determination of phage strains

[0030] Step 1: Collect 10 g of sludge from the surrounding area of ​​an antibiotic enterprise, inoculate it into 100 mL of inorganic salt medium containing 30 mg of penicillin, and culture it on a shaker at 30°C and 160 rpm. Transfer once every 7 days after the start of culture, and transfer to a higher concentration of penicillin-containing medium with a 10% (V / V) inoculum during transfer. The concentration of penicillin is 10mg / L, 30mg / L, 60mg / L, 90mg / L, 120mg / L, and so on, to 900mg / L, to obtain different concentrations of enriched culture solution containing bacteria.

[0031] Wherein, the composition and the proportioning ratio of the inorganic salt culture medium among the present invention are (g / L): K 2 HPO 4 1.6g, KH 2 PO 4 0.4g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.025g, FeCl 3 ·6H 2 O 0.0023g, NH 4 NO 3 0.5g, 20.0g agar, adjust the pH value to...

Embodiment 2

[0042] Example 2: Screening and identification of phage strains and determination of their degradation ability to penicillin

[0043] Step 1: Take 10g of sludge and put it in a sterilized 250mL Erlenmeyer flask, add 100mL of penicillin solution with a concentration of 0.3g / L, and culture on a shaker at 30°C with a rotation speed of 160r / min. After the culture starts, transfer once every 7 days, inoculate into fresh penicillin solution acclimation medium with 10% (V / V) inoculum amount during transfer, and gradually increase the content of penicillin. Detect the degradation rate of penicillin, and select the best group for the isolation of strains.

[0044] Step 2: the bacterial suspension (1 × 10 7 cells / mL) were inserted into 100 mL of tap water containing 30 mg / L penicillin, three parallels were taken, and tap water containing the same concentration of penicillin without inoculating bacteria was used as a control. Cultivate on a shaker at 30° C. and 160 rpm for 2, 4, and 6 ...

Embodiment 3

[0049] Example 3: Screening of phagocytosis bacteria strains and determination of penicillin degradation ability in bacteria residue

[0050] Pass the penicillin waste wet mycelium through a 0.2mm sieve, weigh 10g of the sieved waste mycelium, put it into 100mL of tap water, and add the penicillin waste hyphae obtained after cultivating for 48 hours to the waste wet mycelia solution at about 30°C. Phage 6g (wet weight), 3 groups of parallel experiments were set up, taking the addition of penicillin mycelium but not adding phage phage as a control, taking samples for 0, 2, 4, and 6 hours respectively, and detecting penicillin in the mycelium by HPLC residual amount. The results show that the degradation rate of penicillin can reach 100% after the interaction between phage phage and penicillin hyphae at 30°C for 6 hours.

[0051] Table two KDSPL-04 bacterial strains to the degradation effect of penicillin in the bacteria residue

[0052]

[0053] The beneficial effects of t...

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Abstract

The invention relates to a phagocytic bacterium capable of degrading penicillin, a cell fraction and a composition thereof. The phagocytic bacterium is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee with the preservation number CGMCC No. 12495. The penicillin-degradable greedy phagocytic bacteria, cell fractions and compositions thereof provided by the invention can perform harmless treatment on the penicillin waste mycelium, and have the advantages of environmental protection, energy saving and cost saving.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a greedy phagocytic bacterium capable of degrading penicillin, a cell fraction and a composition thereof. Background technique [0002] Penicillin (Benzylpenicillin) is a general term for a family of antibiotics. It inhibits the synthesis of the cell wall by inhibiting the combination of the tetrapeptide side chain and the pentapeptide cross-linking bridge of the bacterial cell wall. It is a broad-spectrum anti-infective drug. [0003] At present, the industrial production of penicillin mainly uses starch, corn steep liquor, bean cake powder, etc. as raw materials, and is fermented, extracted and purified by Penicillium chrysogenum. After the penicillin aqueous solution is obtained by solid-liquid separation of the fermented liquid, the remaining solid waste residue is waste mycelia. The main components of penicillin waste mycelia are crude protein, cellulose and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28A62D101/26
CPCA62D3/02A62D2101/26A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 刘守信刘文会王鹏黄净范士明田霞
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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