Sepia pharaonis neuropeptide GnRH and use thereof

A technology of tiger squid and neuropeptides, which is applied in the direction of nervous system diseases, specific peptides, peptide/protein components, etc., can solve the problems of tissue preservation that need to be improved, and achieve the prevention of tissue loss of activity, high extraction rate, and prevention of tissue loss. Degradation effect

Inactive Publication Date: 2017-04-26
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has the advantages of simple sequence and strong analgesic activity, but the early tissue preservation needs to be improved, and the extraction process still has room for improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Tiger squid neuropeptide GnRH, the preparation steps are as follows:

[0024] 1) Pretreatment: Dissect the living tiger squid, take the brain tissue in the preservation solution, and store it at ultra-low temperature;

[0025] 2) Take the supernatant: Take the brain tissue and add an equal volume of normal saline, homogenate, centrifuge, take the supernatant A, then add an equal volume of distilled water, centrifuge, take the supernatant B, combine the supernatants A and B to get the above Serum C;

[0026] 3) Column chromatography: The supernatant C is subjected to column chromatography, chromatography, color development, and components are collected to obtain neuropeptides, which are freeze-dried;

[0027] 4) Catalysis: Under the catalysis of ammonium nitrate and metal cadmium, the neuropeptide undergoes a substitution reaction with a GnRH derivative to generate the tiger squid neuropeptide GnRH.

[0028] In step 1, the ultra-low temperature storage temperature is -...

Embodiment 2

[0037] Tiger squid neuropeptide GnRH, the preparation steps are as follows:

[0038] 1) Pretreatment: Dissect the living tiger squid, take the brain tissue in the preservation solution, and store it at ultra-low temperature;

[0039] 2) Take the supernatant: Take the brain tissue and add an equal volume of normal saline, homogenate, centrifuge, take the supernatant A, then add an equal volume of distilled water, centrifuge, take the supernatant B, combine the supernatants A and B to get the above Serum C;

[0040] 3) Column chromatography: The supernatant C is subjected to column chromatography, chromatography, color development, and components are collected to obtain neuropeptides, which are freeze-dried;

[0041] 4) Catalysis: Under the catalysis of ammonium nitrate and metal cadmium, the neuropeptide undergoes a substitution reaction with a GnRH derivative to generate the tiger squid neuropeptide GnRH.

[0042] In step 1, the cryopreservation temperature is preferably -80...

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PUM

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Abstract

The invention provides Sepia pharaonis neuropeptide GnRH and a use thereof. A preparation method of the Sepia pharaonis neuropeptide GnRH comprises 1) pretreatment: taking a living Sepia pharaonis, dissecting to take the brain tissue and storing the brain tissue in a preservation liquid at an ultralow temperature of -70 to -90 DEG C, 2) supernatant collection: taking the brain tissue, adding the equal volume of normal saline into the brain tissue, carrying out homogenization and centrifugation, taking the supernatant A, then adding the equal volume of distilled water into the supernatant A, carrying out centrifugation, taking the supernatant B, and merging the supernatants A and B to obtain a supernatant C, and 3) column chromatography: carrying out column chromatography on the supernatant C, carrying out color development, collecting components and carrying out freeze drying. The preparation method can realize cryopreservation of the tissue, realizes excellent effects, and can effectively prevent the degradation of the Sepia pharaonis brain tissue. The extract can be used in drugs for prevention or treatment on hormone-dependent diseases.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a tiger squid neuropeptide GnRH and its application. Background technique [0002] Tabby squid ( Sepia pharaonis ) are economically important cephalopods that occupy an important position in marine ecosystems. Its meat is fresh and delicious, rich in nutrients, and has potential medicinal value. Regular consumption plays an important role in improving the body's immunity. [0003] GnRH is an ancient and widely distributed reproductive hormone in animals. GnRH plays a very important role in the regulation of reproduction in animals. If the human body lacks GnRH, it will cause a series of body lesions, such as Kallmann Syndrome (KS). Hormonal hypogonadism. However, injecting GnRH or GnRH analogues to farmed animals can reduce the level of related hormones produced by the animal itself, and eventually lose all or part of its fertility. In addition to ...

Claims

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Application Information

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IPC IPC(8): C07K7/23A61K38/09A61P5/24A61P25/00
CPCC07K7/23A61K38/00
Inventor 迟长凤孙连莲曹子豪吕振明徐梅英吴常文
Owner ZHEJIANG OCEAN UNIV
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