Production method of marine animal pesticide

Abstract

The invention discloses a production method of a marine animal pesticide. The production method of the marine animal pesticide in particular comprises the following steps: carrying out pretreatment on raw materials, alcohol extraction, centrifugation, prefiltration, ultrafiltration concentration, salting-out, dissolution, enzymolysis, secondary ultrafiltration concentration, chromatography, third-time ultrafiltration concentration, spray drying and compounding. The whole preparation process is mild in reaction conditions and simple in technological process, and other organic solvents are not used except ethanol; saponins which are combined with proteins and polysaccharides are subjected to precipitation separation by using a salting-out method; furthermore, most of lipids are removed by the salting-out method, so that the production method of the marine animal pesticide is safer and more environment-friendly compared with the traditional method of using organic solvents of ethers and the like to remove the lipids; macromolecular proteins are decomposed into polypeptides and amino acids by immobilized enzymes to effectively reduce adsorption of macroporous resin for the proteins in subsequent processing to increase the amount of a target product in eluent. The starfish saponin content of raw material powder of the animal pesticide is analyzed by using a vanillin-sulfuric acid development process, and is 69.4-73.1%, finally, the marine animal pesticide is obtained through a compounding manner.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and in particular relates to a method for producing animal-derived pesticides by endangering animals in marine breeding industry. Background technique [0002] Starfish (starfish) belong to Echinodermata (Echinodermata), starfish class (Asteroidea). There are more than 2,000 species of starfish, which are distributed in all oceans of the world, and there are more than 100 species of starfish in my country. Starfish feed on a variety of invertebrates or animal carcasses, especially molluscs such as oysters, mussels and clams. They are the enemy of shellfish and coastal aquaculture, and seriously endanger coastal aquaculture fisheries. At present, most of the starfish in shellfish breeding areas are directly discarded or used as crop fertilizer after exposure to the sun and crushed after being caught with the net, which is a kind of pollution to the environment and also causes a waste of resou...

AI Technical Summary

Problems solved by technology

It uses animal-derived bile as an insecticidal component, with low yield, limited sources of raw materials, alkaline formula, and the problem of destroying soil acidity and alkalinity.