Compound leavening agent for special-flavor fermented flour product in southern area of Shanxi province
A seed, aroma-producing yeast technology, applied in the direction of microorganisms, fungi, bacteria, etc., can solve the problems of neglect of scientific and technological talents, few large and medium-sized enterprises, and lack of standardization of enterprises.
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Embodiment 1
[0024] Example 1 Preparation methods of two kinds of aroma-producing yeast
[0025] The two kinds of aroma-producing yeast adopt the same fermentation preparation method, including the following steps:
[0026] ①Seed medium preparation: 2% glucose, 2% peptone, 1% yeast extract, natural pH, sterilized at 115°C for 20 minutes for later use.
[0027] ②Basic medium preparation for fermentation shake flask: 3% glucose, 0.7% yeast powder, KH 2 PO 4 0.1%, K 2 HPO 4 ·3H 2 O0.25%, MgSO 4 0.01%, natural pH, sterilized at 115°C for 20 minutes for later use.
[0028] ③Fermentation tank preparation: After the seeds are cultured, inoculate them into a 5L fermenter containing 3L medium with an inoculation amount of 5% (v / v), adjust the pH of the culture solution to 6.0 with an appropriate amount of acid and alkali, and dissolve oxygen Control, 30%-50%, speed 300r / min (when fermentation broth
[0029] When the dissolved oxygen drops below 40%, increase the rotation speed to increase ...
Embodiment 2
[0030] The preparation method of embodiment 2 acetic acid bacteria
[0031] ① Fermentation medium preparation: 2% glucose, 2% yeast powder, MgSO 4 0.01%, ethanol 4%.
[0032] ②Fermentation tank preparation: After the seeds are cultured, inoculate them into a 5L fermenter containing 3L medium with an inoculum volume of 3% (v / v), set the temperature to 30 °C, the initial ethanol concentration to 4%, and 120 r / min, the dissolved oxygen concentration was maintained at 22%. The fermentation continued for 5 days and was stopped, and the bacteria were collected.
Embodiment 3
[0033] The preparation method of embodiment 3 lactic acid bacteria
[0034] ① Fermentation medium preparation (MRS liquid medium): beef extract 1%, peptone 1%, yeast powder 0.5%, glucose 2%, sodium acetate 0.5%, ammonium citrate 0.2%, K 2 HPO 4 0.2%, MgSO 4 ·7H 2 O 0.05%, MnSO 4 4H 2 O 0.025%, Tween-80 0.1%, pH natural.
[0035] ②Fermentation tank preparation: the fermentation condition is 7% (v / v) of the inoculum, inoculate it in a 5L fermenter containing 3L medium,
[0036] Automatically add 20% ammonia water to maintain the initial pH of the culture, manually add 400 g / L glucose solution to maintain the sugar concentration in the medium at 20 g / L, set the air volume to 0.8 vvm, and the stirring speed to 230 r / min, and cultivate for 30 hours .
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