Method for enriching staphylococcus aureus in milk

Abstract

The invention relates to a method for enriching bacteria, belongs to the technical field of biology, and particularly relates to a method for enriching staphylococcus aureus in milk. The method for enriching the staphylococcus aureus in the milk comprises the following steps of A, collecting a raw milk sample; B, preparing a selective culture medium for staphylococcus aureus enrichment; C, performing separation culture of the staphylococcus aureus, and performing streak inoculation of the collected milk sample to the selective culture medium; D, performing Gram staining microscopy, and extracting a suspected bacterial colony in the selective culture medium, wherein the extracted staphylococcus aureus is Gram-positive cocci; and E, performing enrichment culture of the staphylococcus aureus, transferring the extracted Gram-positive cocci in the step D to a nutrient agar culture medium for performing pure culture, performing enrichment on the staphylococcus aureus, and obtaining the enriched staphylococcus aureus. An economic, quick and specific method for enriching the staphylococcus aureus in the milk is established; and by adopting the method, the staphylococcus aureus in the milk can be quickly, simply and conveniently separated, so that a reliable basis is provided for subsequent identification of the staphylococcus aureus.

Description

technical field [0001] The invention relates to a method for enriching bacteria and belongs to the field of biotechnology, in particular to a method for enriching Staphylococcus aureus in milk. Background technique [0002] At present, milk, as a global drink, is very popular among people. However, there are various flora in fresh milk, and some flora may affect human health. It not only seriously affects the quality of milk products, but even seriously endangers human beings. health, resulting in huge economic losses in dairy farming and the dairy industry. [0003] Therefore, the detection of harmful flora in milk has become a necessary problem to be solved. Due to various reasons, milk may be infected, such as the result of the combined effects of cows being infected by pathogenic microorganisms, trauma, environmental factors, and management factors. When pathogenic microorganisms infect the milk, the milk will contain bacteria that are harmful to the human body. Pathog...

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Problems solved by technology

In GB 4789.18-2010 National Food Safety Standard, in the inspection method of milk and dairy products, milk and dairy products need to be processed. The composition of milk is extremely complex, and some components can interfere with the test results. In order to improve the sensitivity of detection, A suitable enrichment solution can be selected for short-term enrichment culture of the bacteria that may exist in the milk sample, but in terms of ease of operation, labor and identification time, the traditional enrichment culture takes a long time, and generally requires About 5 to 7 days, and the sensitivity of Staphylococcus aureus is low, which is not conducive to the identification of Staphylococcus aureus

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