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Application of carbon nanomaterial to preparation of natural product

A technology of carbon nanomaterials and natural products, applied in the field of preparation of natural products

Active Publication Date: 2017-04-19
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The technical problem to be solved by the present invention is to use graphene oxide to efficiently extract and separate natural products in complex systems with suitable process conditions, so as to realize rapid and accurate qualitative and quantitative analysis of trace components in complex systems

Method used

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  • Application of carbon nanomaterial to preparation of natural product
  • Application of carbon nanomaterial to preparation of natural product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0192] Example 1: Using graphene oxide to separate a standard mixture containing luteolin

[0193] 1. Accurately weigh 0.5 mg each of the standard products luteolin (flavonoids), chlorogenic acid (phenolic acids), Dipsacus saponin B (saponins), and loganin (iridoids), Mix well, dissolve in methanol, and make standard mixtures with concentrations of 0.5 mg / ml.

[0194] 2. Accurately weigh 1mg of graphene oxide and dissolve it in water to form a stable graphene oxide aqueous dispersion.

[0195] 3. Mix the graphene oxide aqueous dispersion with the standard mixed solution evenly, and adjust the pH of the mixed solution with a small amount of NaOH to maintain the pH at 7.

[0196] 4. The mixture was ultrasonically treated for 40 minutes, taken out, centrifuged at 13,000 rpm for 10 minutes, and the supernatant was absorbed to enter the liquid phase for detection.

[0197] 5. Disperse the precipitate in 4 into 1ml of methanol, and adjust the pH of the mixture with formic acid to ...

Embodiment 2

[0206] Embodiment 2: Graphene oxide extracts flavonoids in honeysuckle

[0207] 1. Accurately weigh 0.5g of honeysuckle powder, add 30ml of methanol, heat to reflux for 1.5h, cool to room temperature, make up for the weight loss with methanol, filter, and wait for analysis.

[0208] 2. Prepare two 1.5ml EP tubes, numbered A and B respectively. Accurately weigh 1 mg of graphene oxide in each EP tube, add 800 μl of water, and add 200 μl of honeysuckle extract, the measured pH is 7, and sonicate for 40 minutes.

[0209] 3. Take out the two tubes of mixed solution and centrifuge at 13000rpm for 10min.

[0210] 4. Add 500 μl of acetone:isopropanol (v:v=4:1) mixture to tube A, and add 500 μl of 7% phenol water:acetone (v:v=4:1) mixture to tube B . Make it evenly dispersed, ultrasonic treatment for 40min.

[0211] 5. Take out the two tubes of mixed solution, centrifuge at 13000rpm for 10min, and absorb the supernatant for qualitative analysis by high-resolution mass spectrometry. ...

Embodiment 3

[0231] Example 3: Graphene Oxide Extraction and Quantification of Flavonoids in Lonicerae pilosula

[0232] 1. Accurately weigh 0.5g of the medicinal material Lonicerae pilosula powder, add 30ml of methanol, heat to reflux for 1.5h, cool to room temperature, use methanol to make up the lost weight, filter, and wait for analysis.

[0233] 2. Prepare two 1.5ml EP tubes, numbered A and B respectively. Accurately weigh 1 mg of graphene oxide in each EP tube, add 800 μl of water, and add 200 μl of honeysuckle extract, the measured pH is 7, and sonicate for 40 minutes.

[0234] 3. Take out the two tubes of mixed solution and centrifuge at 13000rpm for 10min.

[0235] 4. Add 500 μl of acetone:isopropanol (v:v=4:1) mixture to tube A, and add 500 μl of 7% phenol water:acetone (v:v=4:1) mixture to tube B . Make it evenly dispersed, ultrasonic treatment for 40min.

[0236]5. Take out the two tubes of mixed solution respectively, centrifuge at 13000rpm for 10min, and absorb the superna...

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Abstract

The invention discloses an application of a carbon nanomaterial to the preparation of a natural product. The natural product in a complex system is efficiently extracted and separated by using the carbon nanomaterial, so that trace components in the complex system can be quickly and accurately subjected to qualitative and quantitative analysis. The method is high in selectivity, is simple and convenient, is efficient, and opens up a new thought for preparing the natural product.

Description

technical field [0001] The present invention relates to the field of preparation methods of natural products, and relates to an extraction method of active ingredients in complex component systems, specifically the use of carbon nanomaterials in the preparation of natural products, using graphene oxide to extract flavonoids from complex component systems. [0002] Background technique: [0003] Graphene oxide is an emerging carbon nanomaterial in recent years, which is widely used in environmental monitoring and repair, dye separation, electrochemical materials and drug release carriers in vivo. The literature proves that graphene oxide has an aromatic hydrocarbon backbone with a delocalized π-electron system, which can be combined with chemical structures with π-π conjugated structures. In addition, graphene oxide is a product modified by graphene functional groups, and its surface has hydroxyl, carboxyl, epoxy and other groups, which can form hydrogen bonds with compounds w...

Claims

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Application Information

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IPC IPC(8): A61K36/355
Inventor 李萍杨华王锐
Owner CHINA PHARM UNIV
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