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Isothermal amplification primer set capable of detecting pork and duck derived components at same time and kit thereof

A source component, constant temperature amplification technology, applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of low efficiency, improve detection efficiency, shorten detection time, reduce detection time, etc. cost effect

Inactive Publication Date: 2017-03-29
HANGZHOU TIANMAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these cross-primer isothermal amplification rapid detection methods can only quickly detect one meat-derived component at a time, and the efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0044] Specific method for simultaneous detection of pork and duck-derived component nucleic acids using the kit of the present invention

[0045] (1) Extract DNA from the sample to be tested: Take a small amount of cut meat sample, add 500 μL sterile double distilled water, cover and shake for 15 seconds, keep warm in a constant temperature water bath at 95°C for 5 minutes, centrifuge and take the supernatant, supernatant spare.

[0046] (2) Reconstitution of the vitrification constant temperature amplification tube: take the vitrification constant temperature amplification tube, add 20ul of reconstitution buffer, then drop 20uL of paraffin oil, and let it stand at room temperature for 2-3 minutes to fully dissolve the vitrification reagent; Then add 3uL of the DNA template solution extracted above, and use a pipette gun to fully blow and mix the liquid in the tube, and then cap the tube tightly.

[0047] Amplification solution is added in the vitrified constant temperature ...

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PUM

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Abstract

The invention discloses an isothermal amplification primer set capable of detecting pork and duck derived components at the same time and a kit thereof. The isothermal amplification primer set comprises (1) a primer used for detecting the pork source component and (2) a primer used for detecting the duck derived component. According to the isothermal amplification primer set capable of detecting the pork and duck derived components at the same time and the kit thereof, the pork derived component and the duck derived component can be subjected to cross primer isothermal amplification at the same time, and by adoption of a nucleic acid test strip, the two meat derived components in samples can be rapidly detected.

Description

technical field [0001] The invention relates to the field of rapid detection of food safety, in particular to a constant temperature amplification primer set capable of simultaneously detecting components derived from pork and duck meat and a kit thereof. Background technique [0002] Meat products are an important source of human protein. In the animal-derived food consumption market, pig, beef, and mutton products are the most popular animal-derived foods for consumers, accounting for the vast majority of daily meat products. However, due to the differences in the quality and price of animal meat from different species, giving Adulteration Adulteration creates profit margins. For example, the price of mutton and beef is much higher than that of pork. At the same time, a lot of leftovers are produced in the processing of chicken and duck, and the price is very cheap. Therefore, in recent years, there have been frequent occurrences of sales of meat from other species mixed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q1/6844C12Q2600/16C12Q2537/143
Inventor 赵澎涛吴东兵廖军华
Owner HANGZHOU TIANMAI BIO TECH
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