Germination-promoting bacillus and application thereof
A technology of Bacillus and Bacillus, which is applied in the field of Bacillus that promotes the growth of Radix Radix, can solve the problems of aggravation of diseases and insect pests, economic loss of pharmaceutical farmers, reduction of yield and quality, etc.
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Embodiment 1
[0083] A kind of bacillus that promotes growth, and described bacillus is isolated and cultivated and obtained like this: specifically comprise the following steps:
[0084] (1) Isolation and screening of autogenous nitrogen-fixing bacteria: Weigh the yellow soil, add 19 times the amount of sterile water, stir for 15 minutes and then let it stand for 10 minutes; take the above supernatant and dilute it to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Soil diluent, then imbibe 10 -3 、10 -4 、10 -5 The soil dilutions were spread on Ashby medium plates with a diameter of 9cm, and each concentration was spread on 3 dishes, and then placed upside down in a constant temperature incubator at 28°C for cultivation. When colonies appeared, the colonies in the repeated dishes were selected and transferred. Put it into a PDA slant test tube, incubate at 25-30°C for 4 days, and set aside;
[0085] (2) Isolation and screening of nitrogen-fixing and potassium-decomposing bacteria: the above-mentio...
Embodiment 2
[0093] A kind of bacillus that promotes growth, and described bacillus is isolated and cultivated and obtained like this: specifically comprise the following steps:
[0094] (1) Isolation and screening of autogenous nitrogen-fixing bacteria: Weigh the mixed soil sample, add 19 times the amount of sterile water, stir for 15 minutes and then let it stand for 10 minutes; take the above supernatant and dilute it to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Soil diluent, then imbibe 10 -3 、10 -4 、10 -5 The soil dilutions were spread on Ashby medium plates with a diameter of 9cm, and each concentration was spread on 3 dishes, and then placed upside down in a constant temperature incubator at 28°C for cultivation. When colonies appeared, the colonies in the repeated dishes were selected and transferred. Into the PDA inclined test tube culture, 4 ℃ constant temperature storage, standby;
[0095] (2) Isolation and screening of nitrogen-fixing and potassium-decomposing bacteria: the abov...
Embodiment 3
[0103] A kind of bacillus that promotes growth, and described bacillus is isolated and cultivated and obtained like this: specifically comprise the following steps:
[0104] (1) Isolation and screening of autogenous nitrogen-fixing bacteria: Weigh the mixed soil sample, add 19 times the amount of sterile water, stir for 15 minutes and then let it stand for 10 minutes; take the above supernatant and dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Soil diluent, then imbibe 10 -3 、10 -4 、10 -5 The soil dilutions were spread on Ashby medium plates with a diameter of 9cm, and each concentration was spread on 3 dishes, and then placed upside down in a constant temperature incubator at 28°C for cultivation. When colonies appeared, the colonies in the repeated dishes were selected and transferred. Into the PDA inclined test tube culture, 4 ℃ constant temperature storage, standby;
[0105] (2) Isolation and screening of nitrogen-fixing and potassium-decomposing bacteria: the above-m...
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