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Pseudomonas aeruginosa and microbial agent thereof, and application of both to passivation of lead

A technology of Pseudomonas aeruginosa and bacterial agents, applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the problems of secondary pollution repair cycle, large engineering quantity, short repair cycle, etc., and achieve high economy Benefits and social benefits, easy operation, and the effect of improving dehydrogenase activity

Active Publication Date: 2017-03-22
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of large amount of work, easy to produce secondary pollution and long repair period in the existing methods for repairing heavy metal pollution, and provide a plant that can be easily operated without pollution and has relatively high Bacterial strain and its application for passivating heavy metal lead with short repair period

Method used

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  • Pseudomonas aeruginosa and microbial agent thereof, and application of both to passivation of lead
  • Pseudomonas aeruginosa and microbial agent thereof, and application of both to passivation of lead
  • Pseudomonas aeruginosa and microbial agent thereof, and application of both to passivation of lead

Examples

Experimental program
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Effect test

Embodiment 1

[0043] This example is used to illustrate the passivation ability of Pseudomonas aeruginosa provided by the present invention to lead in water.

[0044] Inoculate the bacterium solution of the bacterial strain A prepared according to the above preparation example in LB liquid culture medium with an inoculum amount of 1-2% by volume, shake and cultivate at 160rpm and 30°C for 10h, and then add lead into the bacterium solution , so that the initial concentration is 100mg / L. Perform vibration adsorption at 150-170rpm, 28-30°C, take samples every 0.5h, and measure the concentration of lead in the samples.

[0045] Wherein, the detection method of lead content is: take appropriate sample, centrifuge 10min at 8000rpm, take supernatant, use ICP-AES to detect the content of lead in the supernatant, and calculate the removal rate of lead.

[0046] Lead removal rate (%) = (initial concentration of lead - residual concentration of lead) / initial concentration of lead × 100%

[0047] T...

Embodiment 2

[0049] This example is used to illustrate the passivation ability of the bacterial agent provided by the present invention to lead in water.

[0050] The lead in the water body was passivated according to the method in Example 1, except that the lyophilized bacterial cells of strain A prepared according to the above preparation example were added.

[0051] The result is as figure 1 shown.

Embodiment 3

[0065] This example is used to illustrate the promoting effect of Pseudomonas aeruginosa provided by the present invention on the microecology of lead-contaminated soil.

[0066] Add 100 mL of the bacterial solution of strain A prepared according to the above preparation example to lead-contaminated soil with a lead content of 100 mg / kg, stir evenly, and cultivate for 10 days to ensure that the water content is about 20-30%. After 10 days, dry the soil at 65°C, accurately weigh 1g into a 15mL centrifuge tube, add 2mL of 0.1mol / L glucose solution and 2mL of 0.5% TTC solution into the centrifuge tube, mix well, and place at 37°C, 100rpm Incubate in the dark for 16 hours. Then, 1 mL of formaldehyde was added to stop the reaction, and then 5 mL of acetone was added and shaken sufficiently to elute the product TPF attached to the soil particles. Finally, let it stand for 3 minutes, filter the supernatant with an organic filter membrane, measure the absorbance value of the filtrate...

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Abstract

The invention specifically discloses Pseudomonas aeruginosa and application thereof. The accession number of the Pseudomonas aeruginosa is CGMCC No. 10844. The invention also discloses a microbial agent containing the Pseudomonas aeruginosa and a method for passivation of lead. The Pseudomonas aeruginosa provided by the invention has strong capability in passivation of lead in a water body, can finish passivation of lead within 4 to 5 h and has a maximal lead removal rate of 97.33%. As soil contaminated by lead is inoculated with the Pseudomonas aeruginosa, the dehydrogenase activity of the soil can be obviously improved, and lead can be effectively changed into a stable state from a transformable state so as to achieve the effect of lead passivation. Moreover, as the Pseudomonas aeruginosa is used for lead passivation, operation is simple, a period is short, secondary pollution is not posed and high economic benefits and social benefits are obtained.

Description

technical field [0001] The invention relates to a strain of Pseudomonas aeruginosa, in particular to a strain of Pseudomonas aeruginosa with the function of passivating lead and its bacterial agent, and their application in passivating lead. Background technique [0002] At present, the heavy metal pollution of domestic soil is very serious, 16% of the soil is poisoned by heavy metals to varying degrees, and tens of millions of tons of grain are lost every year, which seriously endangers production, life and human health. In recent years, lead poisoning incidents have occurred frequently, especially a series of incidents of children's blood lead exceeding the standard have aroused great concern and extensive discussion in China. Because lead exists widely in the environment, after entering the human body through the respiratory tract and digestive tract, it will cause a series of abnormalities in the nervous system, blood system and digestive system of the body, seriously af...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/385C02F101/20
Inventor 林森李晓桐任立伟王培红
Owner 粮华生物科技(北京)有限公司
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