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Function of tumor progress site 2 in treatment of fatty liver and type 2 diabetes as well as application thereof

A technology for fatty liver and diabetes, applied in the direction of metabolic diseases, digestive system, drug combination, etc., can solve problems such as untreated diabetes

Inactive Publication Date: 2017-03-22
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are no gene regulation and therapeutic agents for the treatment of diabetes

Method used

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  • Function of tumor progress site 2 in treatment of fatty liver and type 2 diabetes as well as application thereof
  • Function of tumor progress site 2 in treatment of fatty liver and type 2 diabetes as well as application thereof
  • Function of tumor progress site 2 in treatment of fatty liver and type 2 diabetes as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] [Example 1] Fatty liver in mice and type Ⅱ diabetes model (diet induced obesity, DIO) were obtained

[0075] (1) Grouping of experimental animals: 8-week-old, male, WT mice and TPL2-KO (TG) mice were selected and given two special feeds, D12942 high fat diet (High fat diet, HFD) and D12450B low fat diet, respectively (Normal chow, NC) feeding, that is, WT NC group, KO NC group, WT HFD group, KO HFD group, a total of 4 groups.

[0076] (2) The model induces the operation process through high-fat feed:

[0077] Using WT and KO (TG) mice, the DIO model was established, and phenotype correlation analysis was carried out to clarify the role of TPL2 gene on fatty liver and type Ⅱ diabetes. Eight-week-old, male, WT mice and TPL2-KO (TG) mice were selected and fed with two special diets, D12942 high-fat diet (Highfat diet, HFD) and D12450B low-fat diet (Normal chow, NC), respectively. That is WT NC group, KO NC group, WTHFD group, KO HFD group, a total of 4 groups. The fasti...

Embodiment 2

[0078] [Example 2] Determination of mouse body weight and blood sugar level

[0079] (1) Fasting body weight and food intake detection of mice

[0080] 1) Weight detection.

[0081] ①Fasting: fast the mice to be tested at 8:00 am (without water), and start the experimental operation at 2:00 pm.

[0082] ② Weighing: Weigh at 0 week, 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, and 12 weeks respectively. In a small bucket, measure the weight and record the data. Feed amount detection: After the weighing operation is completed, add feed to the mice, and record the amount of feed for the mice on the dynamic electronic balance.

[0083] (2) Fasting blood glucose level detection experiment

[0084] All the mice to be tested were fasted from 8:00 am to 2:00 pm (without water), that is, the experimental operation was started after 6 hours of fasting.

[0085] ① Blood glucose meter preparation: Check the battery of the blood glucose meter (Johnson & Johnson, ONETOUCH, USA), press...

Embodiment 3

[0090] [Example 3] Glucose tolerance test (intraperitoneal glucose tolerance test, IPGTT)

[0091] In the 11th week of the experiment, the intraperitoneal injection of glucose test (IPGTT) was performed to evaluate the glucose tolerance of the mice.

[0092] (1) Before measuring blood glucose, measure the fasting body weight of the mice, and calculate the injection volume of glucose based on 10 μL / g.

[0093] (2) First detect the fasting blood glucose at 0 minutes before the glucose injection, and inject the glucose solution intraperitoneally quickly after the detection is completed.

[0094] (3) Operation method of intraperitoneal injection: ①Fix the mouse; grab the mouse, grab the tail of the mouse with the little finger and ring finger of the left hand, and grab the neck of the mouse with the other three fingers, so that the head of the mouse is downward, and the The abdomen of the mouse is fully exposed. ②Needle positioning and injection: insert the needle from the side ...

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Abstract

The invention discloses a function of tumor progress site 2 in treatment of fatty liver and type 2 diabetes as well as an application thereof. According to the invention, TPL2 gene knockout mice and wild type mice are taken as the experiment objects, through a high-fat diet-induced obesity mice model, the result shows that compared with the wild type C 57 mice, body weight of the TPL2 gene knockout mice is mitigated, the fasting blood glucose level is lower than that of a control group WT mice, and the hepatic dysfunction is obviously mitigated. Through intraperitoneal glucose tolerance experiment, the endurance of the TPL2 gene knockout mice on glucose is obviously enhanced. the mice liver tissue cholesterol content change result instructs that TPL2-KO mice fatty liver pathology of a HFD group (High fat diet) is obviously mitigated, and the lipid accumulation is obviously reduced. TPL2 can be taken as a drug target for treating fatty liver and / or type 2 diabetes, and its inhibitor can be used for preparing a drug to treating fatty liver and / or type 2 diabetes.

Description

technical field [0001] The invention belongs to the field of gene function and application, and in particular relates to a tumor progression locus 2 gene (tumorprogress locus 2, TPL2) used as a drug target in screening drugs for the prevention, alleviation and / or treatment of fatty liver and / or type II diabetes application. Background technique [0002] With the increase of aging population, urbanized life and changes in lifestyle, obesity, non-alcoholic fatty liver disease, metabolic syndrome and diabetes and other metabolic abnormalities have increased sharply, and have now become an important hazard to global public health. [0003] Type Ⅱ diabetes mellitus (type 2 diabetes mellitus, T2DM) is non-insulin-dependent diabetes. The etiology is heredity, obesity, high-calorie diet, lack of physical activity and so on. The main manifestation is decreased insulin sensitivity. Diabetes, as a chronic multiple disease, has become a major public health problem of global concern. ...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61P1/16A61P3/10
CPCA61K47/42
Inventor 李红良姬燕晓王丕晓张晓晶
Owner WUHAN UNIV
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