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Method for obtaining new salt-tolerant medicago variety through mutagenesis of loose embryogenic callus

A technology of embryogenic callus and callus, applied in the field of salt-tolerant mutants, can solve the problems of alfalfa salt-tolerant breeding that are rarely reported

Active Publication Date: 2017-03-22
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

[0003] The research on salt tolerance of plants at home and abroad is progressing very rapidly. Much work has been done to screen salt-tolerant mutants of crops by combining mutagenesis technology with in vitro culture. At present, it has been successful in sweet potatoes, tomatoes and other crops. There are few reports on salt-tolerant breeding of alfalfa

Method used

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  • Method for obtaining new salt-tolerant medicago variety through mutagenesis of loose embryogenic callus
  • Method for obtaining new salt-tolerant medicago variety through mutagenesis of loose embryogenic callus
  • Method for obtaining new salt-tolerant medicago variety through mutagenesis of loose embryogenic callus

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example 1

[0029] A method for obtaining a new variety of salt-tolerant alfalfa by mutagenesis of loose embryogenic callus, the steps are as follows: In this example, the mutants of salt-tolerant "favored" alfalfa were screened using the above method

[0030] (1) Preparation of sterile seedlings of alfalfa

[0031] Select plump "favorite" alfalfa seeds, soak them in 70% ethanol for 30s, and then use 40% antiformin aqueous solution for surface disinfection for 15 minutes. The sterilized seeds were repeatedly washed with sterile water for 5 times, each time for 10 minutes, and then the sterilized seeds were inoculated on 1 / 2 MS medium to cultivate aseptic seedlings. Tissue culture seedlings with robust growth can be obtained by culturing at 25°C for 7 days.

[0032] ⑵Induction of alfalfa soft callus

[0033] The hypocotyls of "Faili" alfalfa tissue culture seedlings were used as explants and induced on 1 / 2MS+1.0mg / L2.4-D+20g / L sucrose medium. The culture conditions are: temperature at 2...

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Abstract

The invention discloses a method for obtaining a new salt-tolerant medicago variety through mutagenesis of a loose embryogenic callus; the method comprises the following steps: (1) obtaining a medicago aseptic seedling; (2) inducing a medicago loose callus; (3) inducing a medicago embryogenic callus; (4) inducing the medicago loose embryogenic callus; (5) preparing a mutagenic material; (6) screening a salt-tolerant cell line; and (7) culturing a salt-tolerant mutant strain. The method comprises the main content that the medicago loose embryogenic callus is induced firstly, then the callus is subjected to in-vitro mutagenesis with a chemical mutagen, the medicago loose embryogenic callus after mutagenesis is transferred to a culture medium containing a certain concentration of salt and is subjected to directional screening, the salt-tolerant medicago mutant callus is obtained and is subjected to regeneration culture, and finally the salt-tolerant medicago mutant strain is obtained.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and relates to a method for breeding salt-tolerant mutants by using alfalfa loose-type embryogenic callus under the action of chemical mutagen. Background technique [0002] Alfalfa (Medicago) is one of the most important and widely planted leguminous forages in the world. s King". The alfalfa planting area in the world is about 3.22×107hm2, and the United States, Russia and Argentina account for about 70%. Although China is a big alfalfa planting country, it has never been included in the main body of agricultural production. Alfalfa is regarded as one of the fourth largest crops in the United States, second only to corn, soybean and wheat, with a planting area of ​​about 8.5×106hm2. The planting area of ​​alfalfa in my country is about 3.77×106hm2, ranking first among all kinds of artificial grasslands. In recent years, the breeding of alfalfa is mainly carried out by conventional hy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘艳军杨静慧黄俊轩李双跃武春霞
Owner TIANJIN AGRICULTURE COLLEGE
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