A kind of preparation method of short peptide/silicon dioxide/hyaluronic acid composite hydrogel

A composite hydrogel and silicon dioxide technology, applied in bandages, medical science, etc., can solve the problems of difficult formation of a closed environment on the wound surface, bacterial invasion, etc., and achieve the effects of promoting wound healing, improving mechanical properties, and improving mechanical strength

Active Publication Date: 2019-04-19
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the hydrogel dressing can fit the wound well, it is difficult to form a closed environment with the wound due to non-adhesiveness, and there is still a risk of bacterial invasion

Method used

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  • A kind of preparation method of short peptide/silicon dioxide/hyaluronic acid composite hydrogel
  • A kind of preparation method of short peptide/silicon dioxide/hyaluronic acid composite hydrogel
  • A kind of preparation method of short peptide/silicon dioxide/hyaluronic acid composite hydrogel

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preparation example Construction

[0028] The embodiment of the present invention provides a preparation method of short peptide / silicon dioxide / hyaluronic acid composite hydrogel, including:

[0029] S1: Dissolving the amphiphilic short peptide molecules in water, ultrasonically dispersing them, adjusting to a specified pH value, and placing them at a certain temperature for assembly to obtain self-assembled amphiphilic short peptides;

[0030] In this step, a one-dimensional short peptide self-assembly is constructed using non-covalent interactions between amphiphilic short peptide molecules. Specifically, dissolve the weighed short peptide powder in ultrapure water, disperse it with ultrasonic waves, let it stand for assembly, and ensure that it is fully assembled before using it in the next step. Circular dichroism and infrared spectroscopy can be used to analyze the two peptides. The order structure was observed by atomic force microscope or transmission electron microscope.

[0031] S2: Add the silica pr...

Embodiment 1

[0045] Amphiphilic short peptide I 5 K was dissolved in water and ultrasonically dispersed to form a solution with a concentration of 1 mM, and the pH value was adjusted to 6 to allow assembly at 20°C; tetramethyl orthosilicate (finally) was added to the assembled amphiphilic short peptide solution Concentration 50mM), shake evenly and react at 20°C to obtain short peptide / silica hydrogel; mix the obtained hydrogel with 0.5wt% hyaluronic acid solution to finally obtain short peptide / silica / hyaluronic acid Acid Composite Hydrogel1.

Embodiment 2

[0047] Amphiphilic short peptide A 6 K was dissolved in water and ultrasonically dispersed to make a solution with a concentration of 4mM, and the pH value was adjusted to 5 for assembly at 30°C; tetramethyl orthosilicate and trimethyl orthosilicate were added to the assembled amphiphilic short peptide solution A mixed solution of oxysilane (the molar ratio is 9:1, the total molar concentration is 100mM), after shaking evenly, make it react at 30°C to obtain a short peptide / silica hydrogel; mix the obtained hydrogel with 2wt% The hyaluronic acid solution is mixed to finally obtain the short peptide / silicon dioxide / hyaluronic acid composite hydrogel 2.

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Abstract

The invention relates to an oligopeptide / silicon dioxide / hyaluronic acid composite hydrogel preparation method and belongs to the technical field of medical hydrogel materials. The oligopeptide / silicon dioxide / hyaluronic acid composite hydrogel has the characteristics of improving the strength of hydrogel dressings, having an anti-bacteria function, improving wound healing and being easy to combine with functional molecules. The preparation method comprises the steps of firstly self assembling amphipathic oligopeptide in water solution to form a stable self assembly; mixing silicon dioxide precursor with assembled oligopeptide solution to react in the appropriate condtion to obtain oligopeptide / silicon dioxide hydrogel; compositing the oligopeptide / silicon dioxide hydrogel with hyaluronic acid solution of the certain concentration to obtain the oligopeptide / silicon dioxide / hyaluronic acid composite hydrogel. The preparation method is used for preparing organic / inorganic composite hydrogel with higher mechanical strength, and the organic / inorganic composite hydrogel can be used as a three-dimensional support material of the medical dressings.

Description

technical field [0001] The invention relates to the technical field of medical hydrogel materials, in particular to a method for preparing a silica composite hydrogel that can be used as a wound dressing support material. Background technique [0002] The skin is the physical, chemical and biological barrier between the human body and the outside world. Damage to the skin will destroy this barrier, causing increased metabolism, decreased body temperature, excessive loss of water and protein, and bacterial infection, which can even be life-threatening. Therefore, it is necessary to cover the wound with an appropriate dressing to protect the wound, reduce the risk of infection, and promote wound healing. Based on the traditional theory that a dry environment is beneficial to wound healing, gauze, cotton balls, etc. are the dressings used earlier. Although they have certain moisture absorption and protection effects, they are easy to adhere to the wound and cause secondary dama...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/0061A61L26/0066A61L26/008A61L26/0095C08L89/00C08L5/08
Inventor 王生杰部勇王晓娟夏永清曹美文
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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