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Method for culturing seed tissues and stem tissues of mesembryanthemum crystallinum

A technology of tissue culture and ice vegetables, which is applied in the field of technical regeneration through tissue culture, can solve problems such as difficult control of the salt content of the culture medium, not strong enough plants, and bad taste of ice vegetables, and achieves simple methods, significant effects, and high yield high effect

Inactive Publication Date: 2017-03-15
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, in the large-scale production of ice vegetables, the salt content in the culture medium is not easy to control, and the conventional seed and stem section tissue culture method is used for cultivation, the effect is not ideal, or the obtained plants are not strong enough, or the growth is not good. , the taste of the cultivated ice vegetables is not good

Method used

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  • Method for culturing seed tissues and stem tissues of mesembryanthemum crystallinum
  • Method for culturing seed tissues and stem tissues of mesembryanthemum crystallinum
  • Method for culturing seed tissues and stem tissues of mesembryanthemum crystallinum

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Embodiment 1

[0025] The method for ice vegetable seed tissue culture of the present embodiment, described method comprises the following steps:

[0026] One, the preparation of medium: adjust the concentration of sucrose and agar in the MS medium, then add NaCl in the MS medium, and adjust the pH value to be 5.8, obtain ice vegetable medium;

[0027] 2. Seed treatment: put ice vegetable seeds into a triangular flask, add 40mL water and 2 drops of Tween 80 (surfactant), and shake fully (5min); then rinse ice vegetable seeds with water for 5 times, and drain the water; In the ultra-clean workbench, soak and shake with 75% alcohol for 30 seconds, rinse once with sterile water; soak and shake with 10% sodium hypochlorite solution for 15 minutes, rinse with sterile water for 4-5 times; take out the ice vegetable seeds and put them in In a petri dish with sterile filter paper, blot dry;

[0028] 3. Inoculation and cultivation: take the ice vegetable seeds obtained in step two and place them on ...

Embodiment 2

[0031] The method for ice vegetable seed tissue culture of the present embodiment, described method comprises the following steps:

[0032] One, the preparation of medium: adjust the concentration of sucrose and agar in the MS medium, then add NaCl in the MS medium, and adjust the pH value to be 5.8, obtain ice vegetable medium;

[0033] 2. Seed treatment: put ice vegetable seeds into a triangular flask, add 40mL water and 2 drops of Tween 80 (surfactant), and shake fully (5min); then rinse ice vegetable seeds with water for 5 times, and drain the water; In the ultra-clean workbench, soak and shake with 75% alcohol for 30 seconds, rinse once with sterile water; soak and shake with 10% sodium hypochlorite solution for 15 minutes, rinse with sterile water for 4-5 times; take out the ice vegetable seeds and put them in In a petri dish with sterile filter paper, blot dry;

[0034] 3. Inoculation and cultivation: take the ice vegetable seeds obtained in step two and place them on ...

Embodiment 3

[0037] The method for ice vegetable seed tissue culture of the present embodiment, described method comprises the following steps:

[0038] One, the preparation of medium: adjust the concentration of sucrose and agar in the MS medium, then add NaCl in the MS medium, and adjust the pH value to be 5.8, obtain ice vegetable medium;

[0039] 2. Seed treatment: put ice vegetable seeds into a triangular flask, add 40mL water and 2 drops of Tween 80 (surfactant), and shake fully (5min); then rinse ice vegetable seeds with water for 5 times, and drain the water; In the ultra-clean workbench, soak and shake with 75% alcohol for 30 seconds, rinse once with sterile water; soak and shake with 10% sodium hypochlorite solution for 15 minutes, rinse with sterile water for 4-5 times; take out the ice vegetable seeds and put them in In a petri dish with sterile filter paper, blot dry;

[0040] 3. Inoculation and cultivation: Take the ice vegetable seeds obtained in step two and place them on ...

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Abstract

The invention provides a method for culturing seed tissues and stem tissues of mesembryanthemum crystallinum, relates to a technical regeneration method based on tissue culture, and particularly relates to a tissue culture based technical regeneration method for the mesembryanthemum crystallinum. According to the method for culturing the seed tissues of the mesembryanthemum crystallinum, sprouting ratio is high, and growth tread is good; plant height and fresh weight of the mesembryanthemum crystallinum prepared according to the method are remarkably higher than mesembryanthemum crystallinum prepared according to conventional methods. The method for culturing the seed tissues of the mesembryanthemum crystallinum includes culture medium preparation, seed treatment, inoculation and culture. The method for culturing the stem tissues of the mesembryanthemum crystallinum includes culture medium preparation, inoculation material preparation, inoculation and culture. The method has the advantages that through addition of saline solutions into culture media, mesembryanthemum crystallinum tissue culture seedlings growing well under high saltness (0.9%) conditions are obtained and are applied to salt-resisting production. The method is applied to the field of mesembryanthemum crystallinum planting.

Description

technical field [0001] The invention relates to a regeneration method through tissue culture technology, in particular to a regeneration method for ice vegetables through tissue culture technology. Background technique [0002] With the development of biological science and the increasing degree of salinization of cultivated land and woodland in the world, more and more attention has been paid to the introduction of salt-tolerant varieties and the improvement of plant salt tolerance. , food and other aspects have a high application value. [0003] At present, in the large-scale production of ice vegetables, the salt content in the culture medium is not easy to control, and the conventional seed and stem section tissue culture method is used for cultivation, the effect is not ideal, or the obtained plants are not strong enough, or the growth is not good. , the taste of iced vegetables cultivated is not good. Contents of the invention [0004] The present invention provide...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 荣海燕潘淑芬王坤孙秀润
Owner TIANJIN AGRICULTURE COLLEGE
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