Microbial flora capable of rapidly degrading estrogen and analogs in chicken manure

A microbial flora and rapid degradation technology, applied in the field of microbial engineering, can solve problems such as unclear and complete metabolic pathways, and achieve the effect of improving environmental adaptability and reducing risks to ecology and human health.

Pending Publication Date: 2017-02-22
JILIN BUSINESS & TECH COLLEGE
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Problems solved by technology

Although most of the enzyme genes involved in the metabolism of steroids have been cloned, the metabolic p

Method used

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  • Microbial flora capable of rapidly degrading estrogen and analogs in chicken manure
  • Microbial flora capable of rapidly degrading estrogen and analogs in chicken manure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the cultivation of bacterial classification and the preparation of flora

[0042] Comamonas testosteroni ATCC11996, nine strains of KF1-Fe, Ca3, Cb7, M6, M2, A3, H5 and S19-1, respectively take 200 μL bacterial liquid from the mother liquid of each strain, add 2 mL LB medium. Among them, 40 μg / mL kanamycin sulfate was added to the LB medium of Ca3, Cb7, M6, M2 and A3; 2% NaCl was added to the LB medium of H5 and S19-1. The above-mentioned nine bacterial solutions were cultivated overnight at 27° C. on a shaker at 180 r / min. Use LB medium to adjust the OD of the above nine cultured bacterial solutions 600nm =1. Take the same volume of bacteria solution and mix them to get the composite bacteria group.

Embodiment 2

[0043] Embodiment 2, test of the response characteristics of estrogen in chicken manure degraded by compound flora:

[0044] (1) Degradation of chicken manure in the flora:

[0045] The composite bacterial solution and LB medium were mixed at a volume ratio of 0:1; 1:99; 1:9; 1:0 to prepare a composite bacterial solution with OD values ​​of 0; 0.01; 0.1; 1.

[0046] Take 1 g of chicken manure and add it to the composite bacterial solution with OD values ​​of 0; 0.01; 0.1; 1, and artificially add 0.5 mM estradiol solution, and culture at 27 °C and 180 r / min for 10 days.

[0047] (2) Extraction of estrogen and analogues in chicken manure:

[0048] Since estrogen and its analogues are easily soluble in chloroform, we use chloroform extraction to extract estrogen and its analogues from chicken manure or chicken manure degraded by microbial flora. That is, add 4 mL of sterile water and 1 mL of chloroform respectively, transfer to a centrifuge tube, and shake for 10 minutes. Cent...

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Abstract

The invention discloses microbial flora capable of rapidly degrading estrogen and analogs in chicken manure and belongs to the technical field of microbial engineering. The microbial flora comprises equal amounts of Comamonas testosteroni ATCC11996, KF1-Fe (Comamonas testosteroni ATCC49202), Ca3, Cb7, M6, M2, A3, vibrio H5 and Brucella S19-1. The environment adaptability of strains and the degradation efficiency of the estrogen and analogs are improved under the coordinate symbiosis action of compound microbial flora, and the risk caused by environmental pollution to ecology and human health is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a microbial flora capable of degrading estrogen and its analogues. Background technique [0002] The main sources of estrogen in the environment are the intensive production of chemical production, pharmaceutical industry, agriculture, forestry and animal husbandry. In the past half century, animal husbandry has become a major pollution source of estrogen in the environment (Maser E. et al., J. Steroid Biochem. Mol. Biol., 121, 633-640, 2010). The World Health Organization (WHO) recommended in 2003 that estrogen and its analogues in drinking water should be less than 1 ng / L. my country's Shenzhen and Hong Kong measured the estradiol content of regional water sources, and the results far exceeded the WHO standard (Gelsleichter J. et al., Chemosphere., 63, 1506-1522, 2006). Excessive estrogen in the diet can increase the incidence of tumors in women; it ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/21C02F11/02C12R1/01C12R1/63
CPCC12N1/20C02F11/02C02F2101/305C12N9/0006C12Y101/0105C12Y101/01051C12Y101/01213
Inventor 郭立泉徐淼边红枫马传福张传智熊光明
Owner JILIN BUSINESS & TECH COLLEGE
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