Selective separation medium for caprine mycoplasmal pneumonia subspecies and preparation method of selective separation medium
A technology for separating culture medium and mycoplasma pneumonia, which is applied in the field of veterinary biology, can solve the problem of low isolation rate of pathogenic mycoplasma, and achieve the effect of increasing growth rate and isolation rate
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Embodiment 1
[0021] Embodiment 1 Mycoplasma pneumoniae subspecies selective isolation medium and preparation method thereof
[0022] The composition of selective separation medium: hydrolyzed milk protein 20g, yeast extract powder 55g, glucose 20g, MEM medium 12g, PPLO broth powder 18g, sodium pyruvate 15g, 1% phenol red solution 15mL, healthy horse serum 1500mL, specific Sexual growth inhibitory serum 20ml, deionized water adjusted to 10L;
[0023] The preparation process of specific growth inhibitory serum is as follows:
[0024] Preparation of specific growth inhibitory serum for Mycoplasma ovis pneumonia subspecies:
[0025] New Zealand white rabbits were immunized with the culture of Mycoplasma ovis pneumonia subspecies, and the growth inhibitory titer was not less than 2 6 The specific growth inhibitory serum of Mycoplasma ovis pneumonia subsp.
[0026] The preparation process of Mycoplasma arginini-specific growth inhibitory serum:
[0027] New Zealand white rabbits were immuniz...
Embodiment 2
[0032] Embodiment 2 Mycoplasma pneumoniae subspecies selective isolation medium and preparation method thereof
[0033] The composition of selective separation medium: hydrolyzed milk protein 15g, yeast extract powder 55g, glucose 15g, MEM medium 15g, PPLO broth powder 20g, sodium pyruvate 12g, 1% phenol red solution 15mL, healthy horse serum 1500mL, specific Sexual growth inhibitory serum 20ml, deionized water adjusted to 10L.
[0034] The preparation method of the specific growth inhibitory serum and the selective isolation medium of mycoplasma pneumoniae subspecies is the same as that in Example 1.
Embodiment 3
[0035] Embodiment 3 Mycoplasma pneumoniae subspecies selective isolation medium and preparation method thereof
[0036] The composition of selective separation medium: hydrolyzed milk protein 35g, yeast extract powder 40g, glucose 20g, MEM medium 15g, PPLO broth powder 15g, sodium pyruvate 20g, 1% phenol red solution 18mL, healthy horse serum 1800mL, specific Sexual growth inhibitory serum 20ml, deionized water adjusted to 10L.
[0037] The preparation method of the specific growth inhibitory serum and the selective isolation medium of mycoplasma pneumoniae subspecies is the same as that in Example 1.
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Abstract
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