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Simple preparation method of microorganism particle culture medium

A technology of microbial culture medium and granular culture medium, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsuitable culture medium, etc., and achieves simple preparation method, high sterility rate, and adaptability. wide effect

Inactive Publication Date: 2017-02-15
QINGDAO JIAMING MEASUREMENT & CONTROL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in actual preparation, it is necessary to use a granulator to granulate the powder medium, which can only be done in large factories with special equipment
Moreover, during the granulation process, it is necessary to use hot air to dry the granules, which is not suitable for some media containing heat-sensitive additives

Method used

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  • Simple preparation method of microorganism particle culture medium

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preparation example Construction

[0019] The embodiment of the present invention provides a kind of convenient preparation method of microbial granule culture medium, and specific steps are as follows:

[0020] S1 Prepare each solid reagent for the preparation of microbial culture medium into powder and pass it through a 50-70 mesh standard inspection sieve. After sieving, weigh it out with a balance according to the required distribution ratio of the culture medium and mix it to obtain the culture medium powder.

[0021] In this step, the solids for the preparation of the microbial culture medium are actually prepared into powders and sieved, so that the reagents of different components can achieve a uniform mesh size, which is beneficial to the subsequent preparation of granules.

[0022] S2 Spread the mixed medium powder evenly in a sterile environment, put it into a sterile container after irradiating ultraviolet light for 0.5-2 hours, spray 75% alcohol while stirring the medium powder with a sterile glass ...

Embodiment 1

[0030] Prepare each solid reagent for the preparation of microbial culture medium into powder and pass through a 60-mesh standard inspection sieve. After sieving, weigh out with a balance according to the required medium composition ratio and mix to obtain medium powder;

[0031] Spread the mixed medium powder evenly in a sterile environment, put it into a sterile container after ultraviolet irradiation for 1 hour, spray 75% alcohol while stirring the medium powder with a sterile glass rod, and wet the medium powder After reaching a certain level, filter with a 12-mesh inspection sieve with a large aperture;

[0032] Put the filtered particles into a 40-mesh collection sieve. After filtering through the collection sieve, put the particles retained on the collection sieve into a sterile tray. After 1-2 hours of ultraviolet irradiation, the granule medium is prepared after air-drying;

[0033] The large particles on the large-aperture inspection sieve, the small particles under...

Embodiment 2

[0035] Prepare each solid reagent for the preparation of microbial culture medium into powder and pass through a 50-mesh standard inspection sieve. After sieving, weigh out with a balance according to the required medium composition ratio and mix to obtain medium powder;

[0036] Spread the mixed medium powder evenly in a sterile environment, put it into a sterile container after irradiating ultraviolet light for 0.5h, spray 75% alcohol while stirring the medium powder with a sterile glass rod, and put the medium powder After wetting to a certain extent, filter with a 10-mesh inspection sieve with a large aperture;

[0037] Put the filtered particles into a 35-mesh collection sieve. After filtering through the collection sieve, put the particles retained on the collection sieve into a sterile tray, put the sterile tray into an ordinary ultra-clean bench, and dry it with an ultra-clean bench fan. After 1-2 hours of ultraviolet irradiation, the granule medium is prepared after a...

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Abstract

The invention provides a simple preparation method of a microorganism particle culture medium, and belongs to the field of culture medium preparation. The method can achieve rapid preparation of a small quantity of microorganism particle culture media within several hours. The simple preparation method comprises the main steps that 75% alcohol is sprayed while culture medium powder is added into a sterile container to be stirred, and the culture medium is screened after being wetted to a certain degree to obtain the culture medium with the certain particle degree. The method is simple, and no large equipment is needed; no heating is needed in the particle preparation process, and the activity of thermal sensitive materials in the culture medium can be well kept; the dual sterilization effects of 75% alcohol and ultraviolet are integrated, the culture medium does not need to be sterilized again, and the culture medium can be directly used for culture of microorganisms and other aspects after sterile water is added for redissolution.

Description

technical field [0001] The invention relates to the field of culture medium preparation, in particular to a simple and convenient preparation method for microbial particle culture medium. Background technique [0002] Microbial culture media are widely used in microbial detection and research. Generally speaking, the typical components of microbial culture medium include protein hydrolyzate, inorganic salts, vitamins, trace metals and carbohydrates, etc., and sometimes it is necessary to add some special substances (such as vitamins or cofactors) according to the needs of different microbial growth. and antibiotics). [0003] At present, according to the state of microbial culture medium, it can be roughly divided into liquid medium, powder medium and granular medium. Wherein the liquid culture medium has advantages such as being able to use immediately, but is not conducive to the long-term storage and transportation of the culture medium. As an alternative to liquid med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12R1/19
CPCC12N1/00C12N1/20
Inventor 高心岗张芳朱保超秦杰隋宗斌熊思孟璐
Owner QINGDAO JIAMING MEASUREMENT & CONTROL TECH
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