Microfluidic gel gas-liquid interface smoke exposure device

Abstract

The invention discloses a micro-fluidic gel gas-liquid interface flue gas exposing device. The micro-fluidic gel gas-liquid interface flue gas exposing device comprises an upper-layer chip, a lower-layer chip and a middle-layer chip, wherein the upper-layer chip provides a flue gas inlet and outlet channel; the lower-layer chip provides a cell culture fluid flowing channel; the middle-layer chip is positioned between the upper-layer chip and the lower-layer chip and is sued for loading gel and cells on the gel; cell culture fluid in the lower-layer chip provides a living environment for cells in the middle-layer chip; and the gel in the middle-layer chip becomes a gas-liquid interface and enables the cells to be in direct contact with flue gas. The three layers of chips are combined, the cells are positioned on the gel and do not in direct contact with the cell culture fluid, and direct impact of the culture fluid to the surfaces of the cells is avoided; meanwhile, the culture fluid can be supplied for the cells through the gel, and stability of the living environment of the cells is kept; and on the basis, the cells can be in direct contact with flue gas, so that related biological information of the flue gas stimulating the cells is obtained.

Description

technical field [0001] The invention relates to smoke exposure, in particular to a microfluidic gel gas-liquid interface smoke exposure device. Background technique [0002] For a long time, the health risks of cigarette smoke to human body have been widely concerned. Studies have shown that smokers are more likely to suffer from lung cancer, chronic obstructive pulmonary disease, and acute myocardial infarction than non-smokers. Studies have shown that oxidative stress is a key pathophysiological mechanism in various smoking-related diseases caused by cigarette smoke. Cigarette smoke is a complex mixture, in which oxidative substances can induce the increase of intracellular reactive oxygen species (ROS), and the ROS produced by the body induced by cigarette smoke can cause damage to intracellular proteins, lipids, and DNA. However, the existing evaluation platforms have limited research on its formation mechanism and component hazards. Therefore, it is necessary to deve...

AI Technical Summary

Problems solved by technology

However, such exposure devices are bulky and expensive, and ordinary laboratories cannot afford them, which limits the promotion and popularization of such devices

In addition, due to the characteristics of natural products of cigarettes, there are obvious differences between different production areas and different batches, so the assessment of its toxicity requires a lot of work, and the existing equipment is completely difficult to meet the basic needs of toxicity assessment

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