Building method of Drosophila melanogaster model for screening and researching mitochondria disease virulence gene, and application of same

A genotype, mitochondrial technology, applied in biochemical equipment and methods, microbial assay/inspection, measurement devices, etc., can solve problems such as hindering the application of transgenic mouse models, long life cycle and high cost

Active Publication Date: 2017-02-08
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mice are evolutionarily close to humans, their inherent long life cycle and high cost have seriously hindered the application of transgenic mouse models in the study of mitochondrial diseases

Method used

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  • Building method of Drosophila melanogaster model for screening and researching mitochondria disease virulence gene, and application of same
  • Building method of Drosophila melanogaster model for screening and researching mitochondria disease virulence gene, and application of same
  • Building method of Drosophila melanogaster model for screening and researching mitochondria disease virulence gene, and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Implementation Example 1: Mitochondrial disease-causing gene screening and research establishment of Drosophila model

[0067] Cross dpr-Gal4 (chromosome II) and UAS-mitoGFP (chromosome II) two genotypes of fruit flies, and then select virgin fruit flies containing both transgenes in the progeny, and this virgin fruit fly fruit flies with w 1118 The fruit flies are hybridized, and after the hybridization, the male fruit flies with the darkest eyes are selected among the offspring fruit flies, and then these male fruit flies are separately hybridized with Sco / Cyo (chromosome II) fruit flies, and after the success of the hybridization is confirmed The existence of dpr-Gal4 and UAS-mitoGFP were verified by PCR in male fruit flies, and if the above two transgenes existed at the same time, the offspring of the fruit flies were collected, thus successfully constructing new fruit flies with fluorescent protein-labeled nerve cell mitochondria Strain dpr-Gal4, UAS-mitoGFP / Cyo; ...

Embodiment 2

[0068] Implementation Example 2: Screening of Mitochondrial Disease-causing Genes Using Mitochondrial Disease Research Model

[0069] The characteristics of this screening model are that the shape and quantity of mitochondria in nerve cells are very clear, and the nerve bundles are intact. Through genetic screening, find genes that can disrupt the normal shape and quantity of mitochondria in nerve cells or the integrity of nerve bundles. Its specific implementation process is as follows figure 2 shown. The control fruit flies carrying the RNAi gene were purchased from the Drosophila Strain Center in Bloomington, USA, and the experimental group fruit flies carrying the RNAi gene were purchased from the Drosophila Strain Center of Tsinghua University.

[0070] Specific results are shown in image 3 . Part A is the morphology of the proximal and distal mitochondria of nerve cells in the control group and the experimental group. It can be seen from the figure that the mitocho...

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Abstract

The invention provides a building method of a Drosophila melanogaster model for screening and researching mitochondria disease virulence gene, and an application of the same. In the method, first fluorescent protein is represented by a mitochondria in a wing nerve cell in a Drosophila melanogaster model; second fluorescent protein is represented by in a wing nerve cell film in the Drosophila melanogaster model; the mitochondria is labeled via the first fluorescent protein positioned by the mitochondria; the nerve cell is labeled by the second fluorescent protein positioned by the nerve cell film; and research to mitochondria functions in a nervous system can be achieved in vivo. The Drosophila melanogaster transgene model built by the method can simply and conveniently screen and research mitochondria disease virulence genes; and demands for high flux, strong economic property and short time during mitochondria disease virulence gene screening can be met.

Description

technical field [0001] The invention relates to a model construction method for screening and researching disease-causing genes and its application, in particular to a fruit fly model for screening and researching mitochondrial disease-causing genes and the corresponding construction method and application. Background technique [0002] Mitochondrial diseases are a class of major diseases characterized by abnormal mitochondrial function. The prevalence rate of mitochondrial diseases in newborns in the United States is 1 / 4000. my country currently lacks complete epidemiological data on mitochondrial diseases. There are hundreds or even thousands of cases, suggesting that the disease is not uncommon in China. Mitochondrial disease is a genetic disease. There are more than 100 known mitochondrial disease-causing genes, including genes encoded by mitochondria itself and mitochondrial genes encoded by the nucleus. In addition, there may be many unknown mitochondrial disease genes...

Claims

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Application Information

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IPC IPC(8): A01K67/033C12Q1/02
CPCA01K67/033A01K67/0339A01K2217/15A01K2227/706A01K2267/0306G01N33/5079
Inventor 方燕姗曹旭王海琼王青瑶
Owner SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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