Extraction separation method of fat-soluble metabolite in faeces
A metabolite and separation method technology, applied in the field of extraction and separation of fat-soluble metabolites in feces, can solve the problems of inability to distinguish metabolites, etc., and achieve the effect of simple process and high extraction and separation efficiency
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Embodiment 1
[0022] Example 1 Study on the extraction and separation of metabolites from the feces of rats fed with polymethoxyflavonoids
[0023] The research object of this example is F344 rats fed with 5-hydroxylisotriment. They were raised in a metabolic cage and given standard food for AIN-76A rats (added with a total weight of 0.1% 5-hydroxy lichenpin). The food tank was replenished regularly every day and rat feces were collected within a week.
[0024] In order to conduct in-depth excavation and development of the metabolites in rat 5-hydroxyl chuanxifen feces, the present invention adopts the following extraction and separation methods:
[0025] (1) Crush 4.2g of rat feces samples, extract with 1000ml of organic solvent under reflux for 24 hours, concentrate under reduced pressure and evaporate the solvent; fecal residue is extracted again with the same organic solvent under reflux, and combined with the first extract to obtain the primary Extract the product. The primary extraction p...
Embodiment 2 2
[0030] Example 2 Study on the extraction and separation of metabolites from the feces of mice fed with dihydrochalcone (phlorizin)
[0031] The research object of this example is AJ mice fed epiphloridin. They were housed in a metabolic cage and given standard food for AIN-76A mice (with the addition of 0.1% phloridin in total weight). The food tank was replenished regularly every day and 3.6 g of all rat feces were collected within one week. The extraction and separation method of the present invention is used for the metabolites of phloridin in mouse feces:
[0032] (1) Crush 3.6 g of rat feces samples, and use the three organic solvents of n-hexane, ethyl acetate, and n-butanol to extract 1000ml of each under reflux for 24 hours, and concentrate under reduced pressure to evaporate the solvent; Solvent reflux extraction, combined with the first extract to obtain the primary extraction product. Concentrate, evaporate and weigh to obtain the weight of the primary extraction prod...
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