A method for extracting and separating igm and igg from plasma

A technology for plasma and cold-depleted plasma, applied in the field of extracting and separating IgM and IgG, which can solve the problems of energy consumption, high production cost, and failure to separate IgM protein

Active Publication Date: 2021-09-10
HUALAN BIOLOGICAL ENG CHONGQING +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a method for extracting and separating IgM and IgG from plasma to solve the problem that the low-temperature ethanol precipitation method for separating IgG has low efficiency and does not separate IgM protein. Usually, it needs to be carried out at minus 20°C, which consumes more energy, and ethanol is a flammable and explosive substance. For safety reasons, explosion-proof equipment must also be installed in the workshop, and the production cost is relatively high.

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  • A method for extracting and separating igm and igg from plasma
  • A method for extracting and separating igm and igg from plasma
  • A method for extracting and separating igm and igg from plasma

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Embodiment Construction

[0015] The present invention will be described in further detail below in combination with the accompanying drawings, and specific implementation methods will be given.

[0016] A method for extracting and separating IgM and IgG from plasma. It is characterized in that, comprising the following steps:

[0017] S1. Plasma preparation

[0018] Melt the fresh frozen plasma, centrifuge continuously at 0-3°C to remove the cold glue, and the obtained supernatant is the cold glue-free plasma, and then use a filter element with a pore size of 0.22 μm to filter the cold glue-free plasma. Clarified cold-depleted plasma was obtained. Most of the coagulation factors and lipoproteins are removed by centrifugal precipitation in a low temperature environment, so that the subsequent steps can better extract the required immunoglobulins.

[0019] S2. Preparation for anion exchange chromatography

[0020] The chromatographic filler of anion exchange chromatography is a strong anionic filler...

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Abstract

The invention belongs to the field of protein extraction, and specifically discloses a method for extracting and separating IgM and IgG from blood plasma, adjusting the pH value of the cold glue-free plasma to 6.3-7.0, and adjusting the conductivity of the cold-free plasma to 9-12mS / cm, carry out anion exchange chromatography to obtain the first flow-through solution and the anion gel packing with IgM protein adsorbed; adjust the pH value of the first flow-through solution to 5.4-5.6, and adjust the conductance of the first flow-through solution The rate was 2.0-4.0 mS / cm, and the cation exchange chromatography was carried out to obtain the second flow-through liquid and the cationic gel filler with IgG protein adsorbed thereon. The solution of the present invention can solve the problem of low efficiency of separating IgM and IgG.

Description

technical field [0001] The invention belongs to the field of protein extraction, and in particular relates to a method for extracting and separating IgM and IgG from blood plasma. Background technique [0002] Immunoglobulin is a glycoprotein molecule produced by plasma cells stimulated by antigens under various physiological and pathological conditions. It usually has a Y-shaped structure formed by two heavy chains and two light chains. Immunoglobulins can be divided into IgM, There are five types of IgG, IgD, IgA, and IgE, among which the content of IgG in plasma is 7-16g / L, accounting for 75%-80% of the total immunoglobulin; IgM is extremely strong in the antibody-mediated humoral immune response It is the first antibody produced by the body after anti-infection and immune stimulation. [0003] For the outbreak of novel coronavirus pneumonia since December 2019, the diagnosis and treatment plan issued by the National Health Commission mentions that the diagnostic criteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/06C07K1/18C07K1/14
CPCC07K16/065
Inventor 肖岚刘余江张海梦任虹滕世超张宝献
Owner HUALAN BIOLOGICAL ENG CHONGQING
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