Method of preparing volvaria volvacea essential powder from volvaria volvacea deeply fermented mycelia
A technology of submerged fermentation and mycelium, applied in botany equipment and methods, applications, horticulture, etc., can solve problems such as patent publications that have not been found, and achieve large-scale industrialization, full umami, and increase added value Effect
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Embodiment 1
[0028] A method for preparing straw mushroom fine powder by using straw mushroom submerged fermentation mycelia, the steps are as follows:
[0029] (1) Submerged fermentation mycelium: use straw mushrooms as strains to carry out submerged fermentation to obtain a fermented liquid containing mycelium.
[0030] (2) Mycelium crushing: use a high-speed tissue masher to crush the fermentation broth containing mycelium at a speed of 8000-12000 rpm for 5-20 minutes, and then pass through a colloid mill for homogenization.
[0031] (3) Enzymolysis: Use 1mol / L food-grade hydrochloric acid solution to adjust the pH to 4.5-6.5, add 0.01-0.1% β-glucanase of mycelium weight, enzymolysis at 45-65°C for 1-3 hours, and then use 1mol / L food-grade sodium hydroxide solution to adjust the pH to 6.0-8.0, add 0.01-0.1% protease by mycelium weight, enzymatically hydrolyze at 45-65°C for 1-3 hours, raise the temperature to 85-100°C, and keep for 5-15 minutes. That is, the enzymatic hydrolysis soluti...
Embodiment 2
[0039] A method for preparing straw mushroom fine powder by using straw mushroom submerged fermentation mycelia, the steps are as follows:
[0040] (1) Submerged fermentation mycelium: use straw mushrooms as strains to carry out submerged fermentation to obtain a fermented liquid containing mycelium.
[0041] (2) Mycelium crushing: use a high-speed tissue masher to crush the fermentation broth containing mycelium at a speed of 8000 rpm for 20 minutes, and then pass through a colloid mill for homogenization.
[0042] (3) Enzymolysis: Use 1mol / L food-grade hydrochloric acid solution to adjust the pH to 4.5, add 0.01% mycelium weight β-glucanase, enzymatically hydrolyze at 55°C for 1 hour, and then use 1mol / L food-grade sodium hydroxide Adjust the pH of the solution to 6.0, add 0.01% protease by mycelium weight, enzymolyze at 55°C for 1 hour, raise the temperature to 90°C, and keep for 10min to obtain the enzymatic hydrolysis solution.
[0043] (4) Concentration: filter the enzy...
Embodiment 3
[0050] A method for preparing straw mushroom fine powder by using straw mushroom submerged fermentation mycelia, the steps are as follows:
[0051] (1) Submerged fermented mycelium: take straw mushroom as strain, carry out submerged fermentation, and obtain a fermented liquid containing mycelium.
[0052] (2) Mycelium crushing: Utilize a high-speed tissue masher to crush the fermentation broth containing mycelium, rotating speed: 10000rpm, time 15min, then pass through a colloid mill for homogenization.
[0053] (3) Enzymolysis: use 1mol / L food-grade hydrochloric acid solution to adjust the pH to 5.5, add 0.05% β-glucanase by mycelium weight, enzymolysis at 50°C for 2 hours, and then use 1mol / L food-grade sodium hydroxide Adjust the pH of the solution to 6.5, add 0.01% protease by mycelium weight, enzymolyze at 50°C for 2 hours, raise the temperature to 95°C, and keep for 12min to obtain the enzymolyzate.
[0054] (4) Concentration: filter the enzymolysis solution obtained in...
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