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Mixed fermentation method of biological feed leavening agent

A technology of mixed fermentation and biological feed, which is applied in animal feed, animal feed, food science, etc., can solve the problems of increasing the chance of bacterial contamination, many production links of aerobic bacteria cultivation, and high production costs, so as to reduce the chance of bacterial contamination and reduce the Production links, the effect of reducing production costs

Inactive Publication Date: 2017-01-18
三净生态科技(大连)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that there are many production links for the separate cultivation of aerobic bacteria and anaerobic bacteria, which increases the chance of bacterial contamination and high production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Making A seed liquid: Weigh 1 gram of Lactobacillus plantarum and inoculate it into a 100 ml Erlenmeyer flask containing 100 ml of comprehensive medium, inoculate 5 bottles; culture at 32°C for 72 hours, wrap the mouth of the bottle with plastic, and perform anaerobic culture; Obtain A seed liquid.

[0050] Preparation of seed solution B: Weigh 1 gram of Bacillus subtilis, inoculate it into a 100 ml Erlenmeyer flask containing 100 ml of comprehensive medium, and inoculate 5 bottles; place it in a constant temperature shaker, and cultivate it at 32°C and 150 rpm for 24 Hour, obtain B seed solution.

[0051]The production method of the comprehensive medium is as follows: 20 grams of glucose, 10 grams of corn starch, 1 gram of dipotassium hydrogen phosphate, 0.5 grams of magnesium sulfate, 1 gram of sodium chloride, 1 gram of potassium chloride, 0.01 grams of ferrous sulfate, yeast extract Add 3 grams, 5 grams of peptone, and 10 tablets of vitamin B1 (10 mg per tablet) to...

Embodiment 2

[0064] One, the preparation of seed solution:

[0065] Make A seed solution: weigh 1 gram of Streptococcus thermophilus powder, inoculate into 100 ml Erlenmeyer flasks with 100 ml of comprehensive culture medium, and inoculate 5 bottles; culture at 32°C for 72 hours, wrap the mouth of the bottle with plastic, Oxygen cultivation; A seed solution was obtained.

[0066] Preparation of seed solution B: Weigh 1 gram of Bacillus licheniformis powder, inoculate it into a 100 ml Erlenmeyer flask containing 100 ml of comprehensive medium, and inoculate 5 bottles; place it in a constant temperature shaker, and cultivate it at 32°C and 150 rpm After 24 hours, the B seed solution was obtained.

[0067] The production method of the comprehensive medium is as follows: 20 grams of glucose, 10 grams of corn starch, 1 gram of dipotassium hydrogen phosphate, 0.5 grams of magnesium sulfate, 1 gram of sodium chloride, 1 gram of potassium chloride, 0.01 grams of ferrous sulfate, yeast extract Ad...

Embodiment 3

[0082] Make seed solution A: Weigh 1 gram of Lactobacillus bulgaricus powder and inoculate it into a 100 ml Erlenmeyer flask containing 100 ml of comprehensive medium, inoculate 5 bottles; culture at 32°C for 72 hours, wrap the mouth of the bottle with plastic, and perform anaerobic culture ; Obtain A seed liquid.

[0083] Make B seed solution: Weigh 1 gram of Bacillus cereus powder and inoculate it into a 100 ml Erlenmeyer flask containing 100 ml of comprehensive medium, inoculate 5 bottles; place it in a constant temperature shaker, and cultivate it at 32°C and 150 rpm After 24 hours, the B seed solution was obtained.

[0084] The production method of the comprehensive medium is as follows: take 20 grams of glucose, 10 grams of corn starch, 1 gram of dipotassium hydrogen phosphate, 0.5 grams of magnesium sulfate, 1 gram of sodium chloride, 1 gram of potassium chloride, 0.01 gram of ferrous sulfate, yeast extract 3 grams, 5 grams of peptone, and vitamin B110 tablets (10 mg p...

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Abstract

The invention relates to a method for preparing a feed leavening agent through a mixed fermentation technology, and mainly aims at solving the problems that due to the fact that at preset, aerobic bacteria and anaerobic bacteria are cultured separately, more production links are needed, and the bacterial contamination probability is increased. The method comprises the steps that a seed solution A, a seed solution B and a synthetic culture medium are prepared, and a fermentation tank is disinfected; feeding, disinfecting and inoculating are conducted in sequence, standing is conducted for two hours after inoculating is conducted, an exhaust valve arranged on the fermentation tank is switched on, oxygen is introduced for aerobic culture, the oxygen is introduced for 8 hours, sampling is conducted for a microscopic examination, the growth rates of the aerobic bacteria and the anaerobic bacteria are observed, when the aerobic bacteria reach 75%, oxygen adding is stopped, an air valve is switched off for anaerobic culture for about 8 hours, then a microscopic examination is conducted, when the anaerobic bacteria account for 70%, the air valve arranged on the fermentation tank is switched on for aerobic culture for 8 hours, then a microscopic examination is conducted, when the aerobic bacteria account for 80% or above, oxygen adding is stopped, anaerobic culture is conducted completely, and culture is finished after 48 hours. The method has the advantages of reducing the production links and the bacterial contamination probability.

Description

[0001] Technical field: [0002] The invention relates to a method for preparing a feed starter by using a mixed fermentation process. [0003] Background technique: [0004] The fermentation process of conventional biological feed starter is to cultivate aerobic bacteria and anaerobic bacteria separately, and the same kind of bacteria with the same or similar culture characteristics can be mixed and fermented in the same fermenter. Its shortcoming is that aerobic bacteria and anaerobic bacteria are cultivated separately and there are many production links, which increases the chance of bacterial contamination and high production cost. [0005] Invention content: [0006] The technical problem to be solved by the present invention is to provide a mixed fermentation method of a biological feed starter by mixing and fermenting different aerobic strains, thereby reducing production links, reducing chances of bacterial contamination, and reducing production costs. [0007] The ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12
CPCA23V2400/123A23V2400/169A23V2400/249
Inventor 石连军王振刚卜宗式周凤芹
Owner 三净生态科技(大连)有限公司
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