preparation method and application of C-reactive protein immuno latex microsphere
A technology of reacting proteins and microspheres, applied in the field of medical immunodiagnosis, can solve the problems of low product yield and little effect on antibody activity of coupling efficiency, and achieve the effects of high sensitivity, good clinical application prospects and fast operation.
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Embodiment 1
[0051] The preparation method of C-reactive protein immune latex microspheres provided by the present invention is as follows:
[0052] 1. Prepare polystyrene microspheres with a particle size of 120nm, the concentration is 10%, and take 5ml; the polystyrene microspheres are first diluted with 2-morpholineethanesulfonic acid buffer solution with a concentration of 50mmol / L to a final concentration of 1%. w / v, to obtain a microsphere solution with a pH of 5.5 to 6.5, the volume of the solution is 50 mL;
[0053] 2. Dissolve carbodiimide in 2-morpholineethanesulfonic acid buffer to obtain a carbodiimide solution with a concentration of 10 mg / mL;
[0054] 3. Based on the weight of polystyrene microspheres contained in the microsphere solution, add the carbodiimide solution dropwise to the microsphere solution at a ratio of 50-60 μL per 10 mg of polystyrene microspheres, and add dropwise 250 μL, shake and activate at 4°C for 1 hour at a speed of 50 r / min to obtain an activated po...
Embodiment 2
[0058] The preparation method of C-reactive protein immune latex microspheres provided by the present invention is as follows:
[0059] 1. Prepare polystyrene microspheres with a particle size of 50nm, the concentration is 10%, take 5ml; the polystyrene microspheres are first diluted with 2-morpholineethanesulfonic acid buffer solution with a concentration of 50mmol / L to a final concentration of 0.8% w / v, to obtain a microsphere solution with a pH of 5.5 to 6.5, the volume of the solution is 50 mL;
[0060] 2. Dissolve carbodiimide in 2-morpholineethanesulfonic acid buffer to obtain a carbodiimide solution with a concentration of 10 mg / mL;
[0061] 3. Based on the weight of polystyrene microspheres contained in the microsphere solution, add the carbodiimide solution dropwise to the microsphere solution at a ratio of 50-60 μL per 10 mg of polystyrene microspheres, and add dropwise 240 μL, shake and activate at 8°C for 1 hour at a speed of 30 r / min to obtain an activated polyst...
Embodiment 3
[0065] The preparation method of C-reactive protein immune latex microspheres provided by the present invention is as follows:
[0066] 1. Prepare polystyrene microspheres with a particle size of 200nm, the concentration is 10%, take 5ml; the polystyrene microspheres are first diluted with 2-morpholineethanesulfonic acid buffer solution with a concentration of 50mmol / L to a final concentration of 1.2% w / v, to obtain a microsphere solution with a pH of 5.5 to 6.5, the volume of the solution is 50 mL;
[0067] 2. Dissolve carbodiimide in 2-morpholineethanesulfonic acid buffer to obtain a carbodiimide solution with a concentration of 10 mg / mL;
[0068] 3. Based on the weight of polystyrene microspheres contained in the microsphere solution, add the carbodiimide solution dropwise to the microsphere solution at a ratio of 50-60 μL per 10 mg of polystyrene microspheres, and add dropwise 300 μL, shake and activate at 2°C for 1 hour at a speed of 50 r / min to obtain an activated polys...
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