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A method for comprehensively improving weeding in tobacco fields and disease resistance of tobacco seedlings and reducing teratogenicity

A technology for disease resistance and tobacco seedlings, applied in the field of comprehensively improving the weeding and disease resistance of tobacco fields and reducing teratogenicity, can solve serious problems, alleviate the contradiction of cultivated land, promote the construction of modern tobacco agriculture, and enhance the The effect of anti-disease function

Inactive Publication Date: 2019-12-03
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, with the increase in the frequency of use of quinclorac, the problem of its phytotoxicity has also become more and more serious, and sufficient attention must be paid

Method used

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  • A method for comprehensively improving weeding in tobacco fields and disease resistance of tobacco seedlings and reducing teratogenicity
  • A method for comprehensively improving weeding in tobacco fields and disease resistance of tobacco seedlings and reducing teratogenicity
  • A method for comprehensively improving weeding in tobacco fields and disease resistance of tobacco seedlings and reducing teratogenicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The degradation dynamics of quinclorac in the test soil and water samples basically follow the first-order kinetic equation, that is: Ct=C 0 e －kt , t 1 / 2 =ln2 / k. Table 1 lists the parameters of the quinclorac residue digestion equation in randomly selected soil samples, and the correlation coefficient r between the residual amount Ct of quinclorac in the sample and the degradation time t is all above 0.87. The half-life of quinclorac in the samples ranged from 48.6d to 66.7d.

[0023] Table 1 The residual degradation results of quinclorac in soil samples

[0024]

Embodiment 2

[0026] 1. Determination of Superoxide Dismutase Activity

[0027] Reagent preparation: 0.05mol L -1 Phosphate buffer (pH7.8); extraction medium: 50mmol L -1 pH7.8 phosphate buffer (containing 1% polyvinylpyrrolidone); 130mmol·L -1 Methionine (Met) solution: Weigh 1.399g Met, dissolve it with phosphate buffer and dilute to 100mL; 750μmol L -1 NBT: Weigh 0.06133g NBT, dissolve it with phosphate buffer solution and dilute to 100mL, and store in the dark;

[0028] 100μmol·L -1 EDTA-Na 2 : Weigh 0.037224g EDTA-Na 2 , constant volume 1000mL; 20μmol L -1 Riboflavin solution: Weigh 0.0075g riboflavin, dilute to 100mL, and store in the dark.

[0029] Preparation of enzyme solution: take the fourth piece of unfolded tobacco leaf, remove the midrib, accurately weigh 0.3g (a total of three parts) in a pre-cooled mortar, add 1.5 mL of pre-cooled extraction medium (add in three times, grind once, washed twice), ground into a homogenate in an ice bath, transferred to a 1.5mL centrifu...

Embodiment 3 2

[0081] Effect on tobacco growth and herbicidal effect experiment after embodiment 3 quinclorac treatment

[0082] 1. Observation of experimental symptoms

[0083] Tobacco growth deformity grading standards are formulated according to the different leaf width inhibition rates: if the leaf width inhibition rate is less than 20%, it means that the agent has basically no teratogenic effect on tobacco, which is represented by "0"; Grade I: mildly damaged leaves, new The leaves can be stretched, and the inhibition rate of leaf width is 20%-40%, indicated by "+"; Grade II: moderately damaged, the new leaves can be stretched slightly, and the inhibition rate of leaf width is 40%-60%, indicated by "++" Indicates; Grade III: Severely damaged, the new leaves cannot be stretched normally, and the inhibition rate of leaf width is above 60%, indicated by "+++".

[0084] The main weed controlled by quinclorac is barnyardgrass. In this experiment, the number and fresh weight of barnyardgrass...

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Abstract

The invention discloses a method for comprehensively enhancing tobacco field weeding capacity and disease resisting capacity of tobacco seedlings and reducing malformation, comprising the following steps: detecting content of quinclorac in soil of a tobacco field, and regulating the content of the quinclorac in the soil of the tobacco field to between 1.04*10<-2>mg / kg and 2.08*10<-2>mg / kg, wherein when the content of the quinclorac in the soil is less than 1.04*10<-2>mg / kg, a moderate amount of the quinclorac is applied to the soil; and when the content of the quinclorac in the soil is more than 2.08*10<-2>mg / kg, the tobacco seedlings are transplanted into the tobacco field or active carbon is applied to the soil after the tobacco field is left unoccupied for 48.6-66.7 days. According to the method disclosed by the invention, scientific measures can be taken in a targeted manner according to the content range of residual quinclorac in the soil, so that the residual quinclorac in the soil can be scientifically set within a safe concentration range within which the quinclorac not only plays an excellent weeding role, but also activate disease-resistant active factors of the tobacco seedlings, and meanwhile malformation possibility of the tobacco seedlings is strictly controlled or reduced.

Description

technical field [0001] The invention relates to the technical field of tobacco planting, in particular to a method for comprehensively improving weeding in tobacco fields and disease resistance of tobacco seedlings and reducing teratogenicity. Background technique [0002] Chemical pesticides have played a huge role in the prevention and control of crop diseases and insect pests and in ensuring agricultural harvests, and will continue to play an important role in the process of agricultural modernization. However, in the process of our use of pesticides, if they cannot be used scientifically and rationally, such as excessive application, improper application, and random use, not only will not achieve the purpose of preventing and controlling diseases, insect pests and weeds, but will cause harm to the protected objects. Phytotoxicity, crops cannot grow and develop normally, severe withering and death. [0003] There was a tobacco leaf producing area in Guangdong where quinc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06A01G13/00A01G22/45
CPCA01G7/06A01G13/00
Inventor 陈泽鹏万树青林先丰
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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