Device and method for measuring service life of mono-dispersion up-conversion nanometer fluorescent particles

Abstract

The invention discloses a device and method for measuring the service life of mono-dispersion up-conversion nanometer fluorescent particles. The device comprises a two-photon microscope assembly and an electrical control assembly. The two-photon microscope assembly comprises a laser device, a plurality of optical frames, a Pockels cell, a two-dimensional galvanometer scanning module and a photomultiplier. The laser device emits light beams, the light beams pass through the optical frames and the Pockels cell and enter the two-dimensional galvanometer scanning module to conduct two-dimensional scanning on a sample, and fluorescent signals generated when the sample is excited are collected by the photomultiplier. The electrical control assembly is used for controlling the two-photon microscope assembly to work. By means of the device and method, fluorescent imaging and fluorescent service life measurement are conducted on a UCNPs material in a mono-dispersion state, and data measured through a time-domain method is visual and easy and convenient to process.

Description

technical field [0001] The invention relates to a device and method for measuring the lifetime of monodisperse up-conversion nano fluorescent particles. Background technique [0002] In recent years, the emergence of a variety of fluorescent materials and labeling methods has led to the rapid development of biological microscopy imaging technology. Among them, Up-conversion Nanoparticles (UCNPs), as a new generation of bioluminescent markers, have attracted widespread attention. UCNPs use near-infrared lasers as excitation light, which can effectively avoid the interference of autofluorescence and scattered light of biological samples, and have deep penetration in biological tissues. Its luminescence is still very stable under long-term excitation light irradiation, without flickering, and is not easy to photolysis and photobleaching. In addition, the selected inorganic materials have good chemical stability, low material toxicity, and are not easy to form aggregation in v...

AI Technical Summary

Problems solved by technology

However, due to the long fluorescence lifetime of UCNPs materials, which can generally reach the order of hundreds of microseconds or even milliseconds, the current TCSPC technology cannot measure the fluorescence lifetime of this time order.

At the same time, due to the difference in the microstructure and microenvironment of UCNPs materials in the monodisperse state and the aggregated state, the fluorescence lifetimes are different, so a device that can measure the fluorescence lifetime of UCNPs materials in the monodisperse state and long fluorescence lifetime is needed and method needs to be proposed

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