Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of regenerative repairing cells for treating cerebellar atrophy

A cerebellar atrophy and cell technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of patients who do not use purified cells, and achieve the effect of less impurities, reduced side effects, and improved quality

Inactive Publication Date: 2017-01-04
JIANGSU SUPERBIO LIFE SCI CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the new century, the problem of aging in my country has become more and more prominent, and some senile diseases have begun to increase, and cerebellar atrophy is one of them. Cell transplantation, there is currently no treatment using purified cells injected directly into the patient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The invention discloses a preparation method of regenerative repair cells for treating cerebellar atrophy, comprising the following steps:

[0021] Step A, preparing mesenchymal stem cells: preparing mesenchymal stem cells by selecting a good umbilical cord, cutting, processing tissue, digesting, separating, and culturing;

[0022] Step B, changing the medium of the cells: take the primary cells that have been cultured for more than 24 hours and add new culture medium in proportion to continue the culture;

[0023] Step C, subculture of cells: Observe under a microscope the cells that are covered with a monolayer, which can be subcultured, and cultured for 5 days after subculture, and the medium is changed once during the period;

[0024] Step D, cell expansion: expand the cells in step C under sterile conditions, select cells covered with a dense monolayer for expansion, culture for 4 days and harvest;

[0025] Step E, harvesting cells: digest the amplified cells in s...

Embodiment 1

[0042] 1. Cell preparation:

[0043] Choose a good umbilical cord: one normal newborn umbilical cord by caesarean section, put it in PBS solution containing gentamicin for later use. Cutting: Take out the umbilical cord first, cut the umbilical cord into several sections with scissors, wash it with PBS, wash off the blood stains, wash it twice, and then use ophthalmic scissors to cut the umbilical cord into 1-2mm pieces for easy digestion. Digestion: Pour the shredded tissue pieces into a clean centrifuge tube, add type I collagenase, and the volume of collagenase does not exceed the tissue pieces. Place it in a shaker at 37°C and digest for 100 minutes. Centrifugation: After digestion, place the centrifuge tube containing the tissue pieces in a centrifuge and centrifuge at low speed (500-1000 rpm) for 8 minutes. After centrifugation, discard the supernatant. Cultivation: Blow up the precipitate with a culture solution containing 10% FBS, and add it to two T75 cell culture ...

Embodiment 2

[0057] 1. Cell preparation:

[0058] Choose a good umbilical cord: one normal newborn umbilical cord by caesarean section, put it in PBS solution containing gentamicin for later use. Cutting: Take out the umbilical cord first, cut the umbilical cord into several sections with scissors, wash it with PBS, wash off the blood stains, wash it twice, and then use ophthalmic scissors to cut the umbilical cord into 1-2mm pieces for easy digestion. Digestion: Pour the shredded tissue pieces into a clean centrifuge tube, add type I collagenase, and the volume of collagenase does not exceed the tissue pieces. Place it in a shaker at 37°C and digest for 100 minutes. Centrifugation: After digestion, place the centrifuge tube containing the tissue pieces in a centrifuge and centrifuge at low speed (500-1000 rpm) for 8 minutes. After centrifugation, discard the supernatant. Cultivation: Blow up the precipitate with a culture solution containing 10% FBS, and add it to two T75 cell culture ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of regenerative repairing cells for treating cerebellar atrophy. The preparation method comprises the following steps that a fresh umbilical cord is selected, and mesenchymal stem cells are prepared through the umbilical cord; cell culture is performed; cells are obtained, and the regenerative repairing cells are obtained after cell purification. The regenerative repairing cells obtained by adopting the preparation method are high in purity, low in impurity content, easy to achieve and suitable for small-scale treatment, can improve the quality of in-vivo cells by changing the large environment of human in-vivo cells and makes inactivated cells live again.

Description

technical field [0001] The invention belongs to the technical field of biological in vitro diagnosis, and in particular relates to a preparation method of regenerative repair cells for treating cerebellar atrophy. Background technique [0002] In the new century, the problem of aging in my country has become more and more prominent, and some senile diseases have begun to increase, and cerebellar atrophy is one of them. Cell transplantation, there is currently no treatment that uses purified cells to be injected directly into the patient. Contents of the invention [0003] In view of the above technical problems, the purpose of the present invention is to provide a method for preparing regenerative repair cells for treating cerebellar atrophy. [0004] Technical scheme of the present invention is as follows: [0005] A method for preparing regenerative repair cells for treating cerebellar atrophy, comprising the steps of: [0006] Step A, preparing mesenchymal stem cells:...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61P25/00
CPCC12N5/0668
Inventor 赵钢
Owner JIANGSU SUPERBIO LIFE SCI CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com