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Application of ginsenoside Rg1 in preparation of health product capable of improving kinetism of organisms

A technology of ginsenosides, athletic ability, applied in the field of bioengineering

Inactive Publication Date: 2017-01-04
NORTHEAST NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In fact, there are many potential risks and hazards associated with overdose of certain nutrients

Method used

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  • Application of ginsenoside Rg1 in preparation of health product capable of improving kinetism of organisms
  • Application of ginsenoside Rg1 in preparation of health product capable of improving kinetism of organisms
  • Application of ginsenoside Rg1 in preparation of health product capable of improving kinetism of organisms

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Embodiment Construction

[0014] 1. C2C12 cell culture

[0015] Take out the frozen C2C12 cells from the -80°C refrigerator, quickly transfer to a 37°C water bath to dissolve the cells, centrifuge at 800r for 5 minutes, and discard the supernatant. in DMEM with 10% fetal bovine serum at 37°C, 5% CO 2 in the cell culture incubator. Replace the culture medium with low concentration serum to promote its differentiation. Change the DMEM high-sugar culture medium for culture, and add ginsenoside Rg1 to the culture medium for co-incubation.

[0016] 2. RT-PCR detection of GLUT4mRNA

[0017] After the C2C12 cells were incubated for 24 hours in the medium containing ginsenoside Rg1, the total RNA in the C2C12 cells was extracted using Trizol, and the amount and purity of the extracted RNA were measured by detecting the OD value at 260nm and 280nm. The reverse transcription and amplification experiments were performed according to the RT-PCR kit provided by Takara Company. The primer sequences were as follo...

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Abstract

The invention belongs to the technical field of bioengineering and particularly relates to application of ginsenoside Rg1 in preparation of a health product capable of improving kinetism of organisms. Skeletal muscle contraction is a foundation for the organisms to achieve a kinetism function; the skeletal muscle contraction capacity is influenced by multiple factors, and the energy substance (glucose) extraction efficiency is one of key factors. Glucose uptake is finished mainly by virtue of a glucose transporter 4 on a skeletal muscle cell membrane, so that the gene expression and membrane translocation of skeletal muscle cell GLUT4 are increased, and glucose is a substance basis for skeletal muscle energy intake. Proven by experiments such as RT-PCR and Western blot, the ginsenoside R1g1 is capable of promoting the genetic transcription, protein expression, and membrane translocation of GLUT4skeletal muscle cell GLUT4, so that a health product capable of improving kinetism of organisms can be developed by use of the ginsenoside Rg1.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to the application of ginsenoside Rg1 in the preparation of health care products for improving the body's exercise capacity. Background technique [0002] It has been a long-standing dream of mankind to improve the athletic ability of combatants, rescue and disaster relief personnel, high-intensity sports personnel and animals participating in various competitive competitions. In the past, people mainly improved their athletic ability by supplementing nutrients and strengthening physical training. According to the survey of the United States Olympic Committee in 2004, more than 90% of athletes supplement various nutrients through diet. This phenomenon is also common in other high-intensity physical workers. Nutrients have changed from supplementing sugar and salt before, to supplementing amino acids, creatine and taurine in recent years, and even using steroid ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P39/00A23L1/09
Inventor 霍洪亮贾胜男李凤玉彭雪微王平于清淼孙丽丽
Owner NORTHEAST NORMAL UNIVERSITY
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