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Application of senkyunolide I (SEI) in preparation of medicaments for treating central nervous system neurodegenerative diseases

A kind of ligustilide, neurodegenerative technology, applied in the field of Chinese medicine chemistry, Chinese medicine pharmacology and medicine, can solve the problem of non-specific treatment and so on

Inactive Publication Date: 2017-01-04
SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no specific treatment for this type of disease. Clinically, symptomatic treatment is mainly used to improve and delay the development of the disease.

Method used

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  • Application of senkyunolide I (SEI) in preparation of medicaments for treating central nervous system neurodegenerative diseases
  • Application of senkyunolide I (SEI) in preparation of medicaments for treating central nervous system neurodegenerative diseases
  • Application of senkyunolide I (SEI) in preparation of medicaments for treating central nervous system neurodegenerative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1.SEI is to the influence of BV-2 cell morphology

[0030] The mouse-derived immortalized microglial cell line BV-2 was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. BV-2 cells belong to the semi-suspension and semi-adherent growth cell line. In the resting state, the adherent cell body is small, and there are mostly cell protrusions of different lengths. Most of the protrusions are asymmetrically distributed, and some cells are unipolar or Bipolar, and one end of such cells is usually very long. When the cell density is high, the growth of cell aggregates shows certain characteristics of suspension cell growth; when the cells are stimulated by external stimuli such as OGD / R, the cells are activated. Morphologically, the cell body was enlarged and rounded, the protrusions retracted, and the folds on the cell surface increased. The whole cell was similar to "poached egg" or "amoeba", which was consistent with the literature reports. ...

Embodiment 2

[0031] Embodiment 2.SEI is to the influence of BV-2 cell viability

[0032] The cell viability was detected by the CCK-8 method, and BV-2 cells in the logarithmic growth phase and with a confluence of 70%-90% were taken, and 5×10 4 Cells / mL were seeded in a 96-well plate at a density of 100 μL per well. After 24 hours of normal culture, OGD / R treatment was performed. 10 μL of CCK-8 reagent was added to each well, and the 96-well plate was shaken gently to fully mix the CCK-8. Let it stand in a 37% incubator for 1-4h, and measure the OD value at 450nm in a microplate reader. The calculation formula of Cell Viability is as follows:

[0033] C e l l V i a b i l i t y ( % ) = OD T e s t - OD ...

Embodiment 3

[0036] Example 3. Effect of SEI on mRNA expression level of inflammation-related molecules in BV-2 cells

[0037] The inventors have investigated inflammation-related molecules including pro-inflammatory cytokines (TNF-α, IL-1β, IL-6 and IFN-γ), enzymes (COX-2 and iNOS), cell adhesion molecules (ICAM-1 and VCAM- 1) and the mRNA level of matrix metalloproteinase MMP-9 were semi-quantitatively analyzed, the results are shown in image 3 .

[0038] It can be concluded that OGD 3h / R 6h significantly (P<0.05) increased the mRNA expression levels of the above inflammation-related molecules (except for iNOS and ICAM-1, which were slightly decreased), and the inflammatory induction effect of SEI on OGD / R showed Concentration-dependent inhibition (1-100 μM).

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Abstract

The invention discloses a new medical application of senkyunolide I (SEI), namely application of SEI in preparation of medicaments for treating central nervous system neurodegenerative diseases. The inventor researches the inflammatory reaction inhibiting effect and a possible molecular mechanism of SEI by use of a microglial cell BV-2 cell line in-vitro oxygen glucose deprivation / oxygen-glucose reintroduction inducing activation model; subsequent embodiments and pharmacological research results indicate that SEI is capable of concentration-dependently inhibiting activation of microglial cell IKK / IkBalpha / NF-kB inflammation signal paths induced by oxygen glucose deprivation / oxygen-glucose reintroduction, the neural anti-inflammatory activity points to treatment of central nervous system neurodegenerative diseases relevant to the inflammation signal paths, and especially can be used for effectively preparing medicaments for treating multiple sclerosis, spinocerebellar ataxia and Pick diseases.

Description

technical field [0001] The present invention relates to the fields of Chinese medicine chemistry, Chinese medicine pharmacology and medical technology, in particular to senkyunolide I (senkyunolide I) extracted and prepared from Chinese medicine Ligusticum chuanxiong Hort. For example, new medical uses for multiple sclerosis, spinocerebellar ataxia, and Pick's disease). Background technique [0002] Chuanxiong (Ligusticum chuanxiong Hort.) is a plant of the genus Ligusticum in the family Umbelliferae, and its medicinal part is the rhizome. Chuanxiong is a traditional Chinese medicine in my country. It has the effects of promoting blood circulation and promoting qi, dispelling wind and relieving pain. It was listed as a top grade in the Eastern Han Dynasty "Shen Nong's Materia Medica", and it is a commonly used traditional Chinese medicine for treating depression and migraine. [0003] Phthalides are a class of compounds present in Rhizoma Chuanxiong, which have been shown t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P25/00
CPCA61K31/365
Inventor 梁爽王源冯怡阮克锋房鑫
Owner SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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