Application of quercetin-3-methylether in chemoprophylaxis of esophagus cancer

A technology for chemoprevention and esophageal cancer, which can be used in medical preparations containing active ingredients, organic active ingredients, drug combinations, etc., and can solve problems such as lack of research on isorhamnetol

Inactive Publication Date: 2017-01-04
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on the anti-tumor effect of isorhamnol. Isorhamnol has only been reported in skin cancer and breast cancer. In skin cancer, isorhamnol prevents the transformation of skin cancer cells through its target Erk. process, and it can also inhibit the proliferation of breast cancer cells
However, there is a lack of corresponding research on the relationship between isorhamnol and esophageal cancer

Method used

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  • Application of quercetin-3-methylether in chemoprophylaxis of esophagus cancer
  • Application of quercetin-3-methylether in chemoprophylaxis of esophagus cancer
  • Application of quercetin-3-methylether in chemoprophylaxis of esophagus cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Because isorhamnol may have certain toxicity to cells when it is used in biological applications, it is first necessary to conduct a preliminary evaluation of the toxicity and growth effects of isorhamnol on cells. The relevant experimental procedures are briefly introduced as follows.

[0056] Cytotoxicity test

[0057] The experimental process is: SHEE cells were mixed with 1×10 4 Each well was inoculated in a 96-well plate for culture (10% FBS / DMEM, 37°C, 5% CO 2 ), after 12 hours, replace the fresh medium and add different concentrations of isorhamnol (0 μM, 1 μM , 2.5 μM, 5 μM, 10 μM, 20 μM), after culturing for 24 and 48 hours respectively, add 10 μL CCK- 8 (cell counting kit-8, Japanese colleagues), continue to cultivate for 2 hours, and finally detect the absorbance at 450nm on a microplate reader.

[0058] Experimental results such as figure 1 As shown in A. from figure 1 As can be seen in A, through the detection of the CCK-8 kit, it can be found that 0~1...

Embodiment 2

[0070] The present invention is based on the synchronous construction of animal models of esophageal carcinogenesis, by synchronously adding isorhamnol in the process of NMBA-induced esophageal carcinogenesis, and through the influence of isorhamnol on the model construction process, the effect of isorhamnol on esophageal carcinogenesis Preliminary evaluation of chemoprevention.

[0071] During the experiment, in order to evaluate the effect of isorhamnol on esophageal carcinogenesis, different experimental groups were set up during the construction of esophageal cancer animal model to conduct a preliminary evaluation of the chemopreventive effect of isorhamnol. The specific experimental group settings are as follows: figure 2 As shown, the details are as follows (after the newly purchased rats have adapted to the environment for a week, they will be grouped according to the following groups for the experiment):

[0072] The first group: solvent control group (number of rats...

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Abstract

The invention belongs to the technical field of cancer treatment and prevention, and particularly relates to application of quercetin-3-methylether in chemoprophylaxis of esophagus cancer. The quercetin-3-methylether is used for chemoprophylaxis of a canceration process of esophagus cancer. The overall design idea of the invention is as follows: during an esophageal canceration process of a rat induced with NMBA, through influencing the incidence rate and diversity of precancerous lesion and judging the expression situation of KI67,c-Jun indexes by use of an immunohistochemical method, the chemoprophylaxis effect of the quercetin-3-methylether on esophagus cancer is judged. According to the application, through constructing an esophageal canceration model process of the white rat induced with NMBA, the effect of quercetin-3-methylether on esophagus cancer is specifically evaluated. Results indicate that when quercetin-3-methylether with proper concentration has a favorable effect of preventing the canceration process of esophagus cancer and providing a new concept and reference possibility for chemoprophylaxis of esophagus cancer.

Description

technical field [0001] The application belongs to the technical field of cancer treatment and prevention, and specifically relates to the application of isorhamnol in chemoprevention of esophageal cancer. Background technique [0002] Esophageal cancer is the most common malignant tumor in the world, and ranks sixth among the most common tumors leading to cancer death. The prognosis is very poor, and the five-year survival rate is only 8-12%. Esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) are the two main histological types of esophageal cancer. ESCC is the most common subtype in developing countries, but in the United States and other Western countries esophageal cancer is predominantly EAC. In recent years, although the early diagnosis and early detection technology of esophageal cancer have made great progress, and standard chemotherapy and radiotherapy have been used clinically for decades, the overall survival rate of most clinical advanc...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352
Inventor 董子钢常俊标董子明赵四敏刘康栋李洪林江亚南赵继敏王艳红别荣海
Owner ZHENGZHOU UNIV
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