Special-effect stem-cell in-vitro induction technology
A stem cell and stem cell factor technology, applied in the field of bioengineering, can solve problems such as the unsatisfactory target homing rate of stem cells and the low utilization rate of stem cells.
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[0010] The present invention will be described in detail below in conjunction with specific embodiments.
[0011] A technique for in vitro induction of special-effect stem cells, which specifically includes the following steps: 1) Isolation and cultivation of human umbilical cord stem cells: In this experiment, human umbilical cord stem cells were used, human umbilical cord Wharton's jelly was taken aseptically, and DMEM culture medium containing 12% FBS was used repeatedly Rinse Wharton's jelly, collect the cell suspension, and change the medium once every 24 hours and 48 hours of the primary culture; and then primary culture for about 7 days, if the cells are observed to form sheets, digest with 0.3% trypsin and passage to a new bottle , after standing in the incubator for 40 minutes, turn the bottom of the bottle up, blow gently, and discard the suspended cells (mainly epithelial cells), while most of the human umbilical cord stem cells firmly adhere to the wall; add the who...
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