Culture method of selenium-rich high-cordycepin Cordyceps sinensis mycelia
A technology of cordycepin mycelium and high cordycepin, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of low content of cordycepin and low content of selenium, and achieve high content of selenium element , high labor efficiency and advanced methods
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[0033] A method for cultivating Cordyceps mycelium rich in selenium and high in cordycepin content, comprising the following steps:
[0034] (1) Activation of strains: Inoculate the strains of Cordyceps militaris in the following medium for activation and optimization: glucose 15-20 grams, magnesium sulfate 1-2 grams, potassium dihydrogen phosphate 1-2 grams, vitamin B10.1 -0.5 mg, 45-55 grams of white sea cucumber grinding liquid, 3-5 grams of yeast extract powder, 15-20 grams of agar powder, add water to 1000 ml; sterilize the above nutrient solution at 121 ° C, 0.1 MPa for 30 minutes, After cooling down, pour it into a petri dish; put the Cordyceps militaris strains into the petri dish, and cultivate it in the dark at 22°C for 5-10 days, select colonies with fast growth, dense hyphae, and pure color, and then transfer them to the slope Cultivate on the medium for 5-10 days in the dark at 22°C, and the resulting strain is the desired Cordyceps strain;
[0035] (2) Preparati...
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