A method for rapid separation of oyster peptides from oyster hydrolyzate

Abstract

The invention discloses a method for quickly separating oyster peptide from oyster enzymolysis liquid. The method comprises the following steps: adding an emulsifier and lipase into enzyme-deactivated oyster enzymolysis liquid, uniformly mixing, and performing hydrolysis at the temperature of 35 to 50 DEG C for 4 to 8 h to obtain a secondary enzymolysis product of oyster; adding yeast cell wall polysaccharides and active carbon into the secondary enzymolysis product, oscillating during heat preservation at the temperature of 50 to 60 DEG C for 0.5 to 1.5 h, performing vacuum suction filtration through a filter membrane of 0.5 [mu]m, and collecting filtrate to obtain the oyster peptide. According to the method, the lipase is used for hydrolyzing fat in an enzymatic hydrolysis system to break the emulsification balance in the enzymatic hydrolysis system and drive a water phase to be separated from an oil phase. The hydrolysis conditions of the method are mild, and the process is safe and controllable; large-scale industrial production can be realized.

Description

technical field [0001] The invention belongs to the field of deep processing of oysters, in particular to a method for rapidly separating oyster peptides from oyster enzymatic hydrolyzate. Background technique [0002] Oysters are the largest cultured shellfish in the world and one of the four largest marine cultured shellfish in my country. They are distributed in various coastal provinces. Oysters are not only rich in nutrition, but also have the characteristics of fresh taste and good efficacy, and are loved by consumers. [0003] Oyster meat is rich in protein (45-57%), fat (7-11%) and glycogen (19-38%), in addition, it also contains sufficient vitamins and minerals such as zinc, calcium, selenium and iron. Oyster protein is a high-quality protein, rich in amino acids, and the completeness and quality ratio of essential amino acids are better than human milk and cow milk. In addition to the 20 commonly used amino acids necessary for the human body, it also contains β-am...

AI Technical Summary

Problems solved by technology

However, in actual production, there are great difficulties in the separation and extraction of oyster peptides

The oyster protein enzymatic hydrolyzate contains complex components such as polypeptides, undegraded proteins, oils and sugars, forming a stable emulsified system, and the separation effect of centrifugation or suction filtration alone is not good.

When centrifugation is used for separation, the interface between the water phase and the oil phase of the oyster protein enzymatic hydrolyzate is unclear and difficult to separate, which seriously affects the separation of the enzymatic hydrolyzate and the yield of the target peptide is not high

However, it takes too long to separate oyster peptides by suction filtration, which is prone to deterioration during processing and has low production capacity.

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