A kind of test tube and method for centrifugal pretreatment of saliva dna

Abstract

The invention discloses a test tube and a method applied to saliva DNA centrifugal pretreatment. The test tube comprises a test tube body, a test tube cap and a seal cap, wherein a protrusion around the periphery of the bottom of the test tube body is arranged at the bottom of the test tube body or a protrusion around the tube wall of the test tube body is arranged at the lower portion of the test tube body, and a seal connection mechanism is arranged at the tube opening position of the test tube body; the test tube cover is matched with and in seal connection with the seal connection mechanism through a seal connection mechanism, and the cells which are obtained from centrifugal treatment and contain the saliva DNA can precipitate in the test tube cap when the centrifugal treatment is performed with the pipe opening of the test tube body downward; the seal cap is matched with and in seal connection with the seal connection portion through a seal connection structure, and the test tube cap can be sealed to be used for storing the cells which are obtained from centrifugal treatment and contain saliva DNA. The saliva DNA is concentrated into a half-solid state which is small in size and stored in the test tube cap and the seal cap, the carrying size is small, great convenience is brought to storage and transportation of saliva samples in a room temperature, and the DNA quality isn't affected.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a test tube and method for centrifugal pretreatment of saliva DNA. Background technique [0002] DNA can theoretically be obtained from any cell in the body. The epithelium of human oral mucosa is stratified squamous epithelium, which has the characteristics of vigorous metabolism, rapid renewal and easy shedding. It consists of multiple layers of cells, the basal layer has a strong ability to divide, and the superficial keratinized layer has been aging and degraded, and continues to fall off. Keratinocytes can be shed naturally into saliva, and can also be detached from the carrier under external force. Although these exfoliated oral cells have condensed nuclei, they also have a complete human genome DNA sequence. It has been reported that PCR reactions were performed using DNA from oral mucosal epithelial cells and peripheral blood respectively, and the re...

AI Technical Summary

Problems solved by technology

[0003] The traditional method of adding a stabilizer to saliva and putting it into a test tube has two obvious defects: (1) the test tube is easily broken and occupies a large volume; (2) the liquid substance is inconvenient to transport and cannot be carried on the plane

However, the test strip has at least two limitations: (1) it is expensive, with a piece of test strip exceeding US$10; (2) the saliva sample that the test strip can collect is very limited

Also existing freeze-drying technology needs huge and expensive equipment, the cost is higher, and the operation is complicated

Images