Method for anhydrously surviving freshwater fish through repeated dormancy
A technology for keeping freshwater fish alive without water, which is applied in fish farming, application, climate change adaptation, etc. It can solve the problems that do not involve repeated dormancy, so as to facilitate the transportation of living organisms, improve the survival rate, and keep alive without water The effect of prolonged
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Embodiment 1
[0017] A number of crucian carp were selected from the Chaimi River Basin in Huai’an City, with an average body length of 15.5±1.5cm and an average weight of 54.2±10.0g. They are healthy and active, uniform in size, complete in scales, healthy and disease-free; The water is tap water inflated by an air pump for at least 24 hours; then, transfer the live fish to the edible alcohol diluent to induce dormancy, and keep them alive for 12 hours at 5°C without water; the alcohol content of the edible ethanol-containing diluent is public The ethanol-containing diluent in the patent "Method for keeping freshwater fish alive without water" CN101107922A; then, revive in clean water at room temperature, rest for 50 hours; secondly, transfer to edible ethanol diluent to induce the second dormancy, and place it at 5°C Keep alive without water for 12 hours; finally, resuscitate in clean water at room temperature.
[0018] Using reverse high-performance liquid chromatography, the change of l...
Embodiment 2
[0022] Select a number of crucian carp from the Chaimi River Basin in Huai’an City. The body length of crucian carp is 10-12cm, and the weight is 100-150g; first, stop feeding in clean water at room temperature for 35 hours, and the temporary breeding water is tap water inflated by an air pump for at least 24 hours; Put it into the liquor diluent, induce dormancy, and keep it alive without water at 12°C for 8 hours; wherein, the alcohol content of the ethanol-containing liquor diluent is the ethanol-containing diluent in the published patent "Method for keeping freshwater fish alive without water" CN101107922A ; Then, revive in clean water at room temperature, rest for 27 hours; secondly, transfer to liquor diluent to induce the second dormancy, and keep alive for 8 hours at 12°C without water; finally, revive in clean water at room temperature.
[0023] Repeated this many times, since the first induction timing, the ultimate survival time of crucian carp can reach 126 hours. ...
Embodiment 3
[0025] Select a number of crucian carp from the Chaimi River Basin in Huai'an City. The body length of crucian carp is 10-12cm, and the weight is 100-150g; first, stop feeding in clean water at room temperature for 20 hours, and the temporary breeding water is tap water inflated by an air pump for at least 24 hours; Put it into the liquor diluent, induce dormancy, and keep it alive without water for 5 hours at 20°C; wherein, the alcohol content of the ethanol-containing liquor diluent is the ethanol-containing diluent in the published patent "Method for keeping freshwater fish alive without water" CN101107922A ; Then, revive in clean water at room temperature, and rest for 5 hours; secondly, transfer to liquor diluent to induce the second dormancy, and keep alive for 5 hours at 20°C without water; finally, revive in clean water at room temperature.
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