A high-precision biocomposite 3D printing device and printing method

Abstract

A high-precision biocomposite 3D printing device and printing method, the device includes a cell printing nozzle connected to a motion control module and an electrostatic direct writing device on the collection platform, the cell printing nozzle is connected to an ultra-precision syringe pump, and an electrostatic direct writing device It is connected with the reflow heating device, and the ultra-clean test bench is equipped with a host, a high-voltage DC power supply, an electrostatic direct writing device, a cell printing nozzle, and a high-voltage DC power supply are all connected to the host; the printing method automatically switches the cell printing nozzle and the electrostatic direct writing device through the host , to print a composite structure of gel / biological scaffold / microchannel containing cells. After printing one layer, the cell printing nozzle and electrostatic direct writing device move the distance of one layer along the Z-axis to print the next layer, and so on. Until the printed tissues and organs are all completed, the present invention can stably and accurately print the complexes of various cells and submicron or nanoscale biological scaffolds.

Description

technical field [0001] The invention relates to the technical field of biological 3D printing, in particular to a high-precision biological composite 3D printing device and printing method. Background technique [0002] Bio-3D printing technology is the integration of 3D printing technology in the engineering field into tissue engineering, involving clinical medicine, biomaterials, biological cytology, etc. This technology uses 3D printing technology to manufacture tissues and organs in vivo or in vitro, which provides technical means for the manufacture of artificial tissues and organs, and is the frontier and hot spot of biomanufacturing technology. At present, the main direction of biological 3D printing technology is to study new cell printing methods, bio-scaffold printing methods, and improve molding accuracy and molding mechanical properties. There are many ways to print cells and bio-scaffolds, but there are still many shortcomings in the existing technology in the ...

AI Technical Summary

Problems solved by technology

There are many ways to print cells and bio-scaffolds, but there are still many shortcomings in the existing technology in the construction of large-scale tissues and organs: 1) Cell printing is usually printed by mixing cells and gel materials, and the mechanical strength of the formed artificial tissue or organ Poor, it is difficult to form large tissues and organs with complex internal microstructure; 2) The distribution of cells inside the biological scaffold is not uniform, which is not conducive to the development and functional expression of corresponding organs; 3) The printing accuracy of biological scaffolds is limited, the microscopic scale of the scaffold Cells are very different, it is difficult to provide a micro-scale microstructural environment for cell adhesion and growth

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