High-precision bio-compound 3D printing device and printing method

Abstract

The invention discloses a high-precision bio-compound 3D printing device and a printing method. The high-precision bio-compound 3D printing device comprises a cell printing spraying head and an electrostatic direct writing device which are arranged above a collection platform and are connected with a motion control module, wherein the cell printing spraying head is connected with an ultra-precision injection pump; the electrostatic direct writing device is connected with a reflux heating device; a main unit and a high-voltage direct current power supply are arranged outside an ultra-clean experiment table; the electrostatic direct writing device, the cell printing spraying head and the high-voltage direct current power supply are connected with the main unit. The printing method comprises the following steps: the main unit automatically switches the cell printing spraying head and the electrostatic direct writing device to print a cell-containing gel / biological scaffold / micro-channel compound structure; after the printing of one layer is completed, the cell printing spraying head and the electrostatic direct writing device move a distance equal to the thickness of one layer in the Z-axis direction for printing of the next layer, and repeating till all tissue organs to be printed are completed in printing. The high-precision bio-compound 3D printing device can stably and precisely print compounds of various cells and submicron or nano-level biological scaffolds.

Description

technical field [0001] The invention relates to the technical field of biological 3D printing, in particular to a high-precision biological composite 3D printing device and printing method. Background technique [0002] Bio-3D printing technology is the integration of 3D printing technology in the engineering field into tissue engineering, involving clinical medicine, biomaterials, biological cytology, etc. This technology uses 3D printing technology to manufacture tissues and organs in vivo or in vitro, which provides technical means for the manufacture of artificial tissues and organs, and is the frontier and hot spot of biomanufacturing technology. At present, the main direction of biological 3D printing technology is to study new cell printing methods, bio-scaffold printing methods, and improve molding accuracy and molding mechanical properties. There are many ways to print cells and bio-scaffolds, but there are still many shortcomings in the existing technology in the ...

AI Technical Summary

Problems solved by technology

There are many ways to print cells and bio-scaffolds, but there are still many shortcomings in the existing technology in the construction of large-scale tissues and organs: 1) Cell printing is usually printed by mixing cells and gel materials, and the mechanical strength of the formed artificial tissue or organ Poor, it is difficult to form large tissues and organs with complex internal microstructure; 2) The distribution of cells inside the biological scaffold is not uniform, which is not conducive to the development and functional expression of corresponding organs; 3) The printing accuracy of biological scaffolds is limited, the microscopic scale of the scaffold Cells are very different, it is difficult to provide a micro-scale microstructural environment for cell adhesion and growth

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