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Predictive biomarker for human colorectal cancer or human colorectal cancer metastasis and applications of predictive biomarker

A biomarker, colorectal cancer technology, applied in the field of predictive biomarkers, ubiquitin-conjugating enzymes, can solve problems such as unclear mechanism of action

Active Publication Date: 2016-12-07
SUZHOU UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the role of Ube2v1 in tumors including colorectal cancer and its mechanism are still unclear.

Method used

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  • Predictive biomarker for human colorectal cancer or human colorectal cancer metastasis and applications of predictive biomarker
  • Predictive biomarker for human colorectal cancer or human colorectal cancer metastasis and applications of predictive biomarker
  • Predictive biomarker for human colorectal cancer or human colorectal cancer metastasis and applications of predictive biomarker

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Embodiment Construction

[0043] Below in conjunction with specific embodiment, further illustrate the present invention, should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention, after having read the present invention, those skilled in the art will understand various equivalent forms of the present invention All modifications fall within the scope defined by the appended claims of the present application.

[0044] 1. Construction of colorectal cancer cell lines with stable overexpression of Ube2v1

[0045] Digest the cells, culture them in a 6-well plate for 24 hours, add an appropriate amount of lentivirus and culture them for 24 hours, change the medium, continue to culture for 24 hours, and observe the fluorescence expression under a fluorescence microscope. Cells were selected by adding purine at a concentration of 1:1000. Western blotting was used to identify whether the lentivirus transfection...

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Abstract

The invention discloses a predictive biomarker for human colorectal cancer or human colorectal cancer metastasis. The predictive biomarker comprises Ube2v1 protein. Based on the constructed colorectal cell strain with Ube2v1 over-expressed stably, the naked mouse experimental result shows that Ube2v1 can promote the growth and metastasis of tumor. Ube2v1 without functions is bonded to Ube2N for degrading histone deacetylase Sirt1, so that the deacetylation effect of Sirt1 for the H4K16 site of the histone is weakened, and the metastasis of colorectal cancer is promoted. The NSC697923 compound is added in the colorectal cancer cells, and the degradation effect of Ube2v1 for the histone deacetylase Sirt1 is obviously weakened. The result shows that Ube2v1 can be taken as the marker for the generation and metastasis of colorectal cancer, and in addition, NSC697923 can be used for preparing medicines for resisting colorectal cancer metastasis, thus having a significant clinical application value.

Description

technical field [0001] The invention relates to an ubiquitin-conjugating enzyme, in particular to human colorectal cancer or human colorectal cancer metastasis predictive biomarker Ube2v1 and its new application, belonging to the field of biomedical technology. Background technique [0002] The ubiquitin-proteasome pathway consists of ubiquitin (ubiquitin, Ub), ubiquitin-activating enzyme (ubiquitin-activating enzyme, E1), ubiquitin-conjugating protein (ubiquitin-conjugating protein, E2), ubiquitin-protein ligase (ubiquitin-protein ligase, E3), 26S proteasome and ubiquitin resolving enzymes (deubiquitinating enzymes, DUBs), etc., the degradation of the target protein is a cascade reaction in which each component undertakes the corresponding function. In short, ubiquitin first obtains activity under the catalysis of E1, and the E1-ubiquitin-bound intermediate transfers ubiquitin to E2 to form an E2-ubiquitin intermediate; finally, E3s can directly or indirectly bind to the su...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/574G01N33/573A61K31/436A61K31/715A61K31/345A61P35/00
CPCA61K31/345A61K31/436A61K31/715C12Q1/6886C12Q2600/136C12Q2600/158G01N33/573G01N33/57419G01N2333/9108
Inventor 李建明沈彤蔡令栋李师
Owner SUZHOU UNIV
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