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Bacillus licheniformis and applications thereof in kitchen waste

A technology of Bacillus licheniformis and kitchen waste, applied in the field of microorganisms, can solve the problems of low efficiency and poor effect, and achieve the effect of good treatment effect, good development prospects, efficient, fast and stable treatment method

Active Publication Date: 2016-12-07
BIOMAX ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing biological treatment methods have low efficiency and poor effect

Method used

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  • Bacillus licheniformis and applications thereof in kitchen waste
  • Bacillus licheniformis and applications thereof in kitchen waste
  • Bacillus licheniformis and applications thereof in kitchen waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and screening of Bacillus licheniformis (Bacillus licheniformis) CY-1

[0033] The separation process of Bacillus licheniformis (Bacillus licheniformis) CY-1 of the present invention: take by weighing 10g of radish rhizosphere soil planted in Jiangning District, Nanjing, add 90mL sterile water, get 10 -1 The diluted solution was cultured in a shaker at 30°C at 130r / min for 2 to 3 hours, and then diluted with sterile water to obtain 10 -5 、10 -6 、10 -7 、10 -8 200uL of the above dilutions were spread on LB plates, cultured at 30°C for 48 hours, and a single colony was picked and inoculated on the slant of LB solid medium for preservation.

[0034] The physiological activity characteristics of Bacillus licheniformis CY-1 are as follows:

[0035] a. Morphological characteristics: Gram staining is positive, spores are produced, the spores are oval, located in the middle of the bacteria or slightly biased; there are perinatal flagella, and can move; th...

Embodiment 2

[0041] Example 2 Preparation of Biological Treatment Agent Containing Bacillus licheniformis Inoculum and Application in Kitchen Waste

[0042] Preparation of biological treatment agent containing Bacillus licheniformis:

[0043] (1) Culture Bacillus licheniformis on LB solid culture at 30°C for 24h; inoculate it in a fermentation liquid shaker flask, shake it at 30°C and 130r / min for 24h to form a seed liquid;

[0044] The seed liquid is inoculated into the culture material in the fermenter at a volume ratio of 1:400 for fermentation production. The basic working parameters of the fermenter are: temperature: 30-35°C, pH=6.6-7.2, stirring speed: 130r / min , dissolved oxygen: 30%, tank pressure: 0.04MPa; stop the fermentation after 24 hours of fermentation, then centrifuge at 6000r / min for 10min, take the sediment and dilute it with sterile water to make a bacterial agent; the concentration of viable bacteria in the bacterial agent is detected to be 5.6 ×10 9 CFU / mL;

[0045] (...

Embodiment 3

[0055] Example 3 Preparation of Biological Treatment Agent Containing Bacillus licheniformis Inoculum and Application in Kitchen Waste

[0056] This embodiment is basically the same as Example 2, except that in step (2), the mass ratio of sawdust, bran powder and rice husk is 7:2:1; in step (3), microbial inoculum and carrier The mass ratio is 1:13.

[0057] Weight loss efficiency test of biological treatment agent on food waste after treatment:

[0058] (1) The composition of kitchen waste: the food left in the canteen, remove the large and hard parts such as plastic or bones;

[0059] (2) The biological treatment agent prepared by the above method is applied to the treatment of kitchen waste: preheating operation 6h, ventilation and stirring at the same time; 10kg of food waste to be treated is added in the processor after the pre-operation, and biological treatment agent Mix for aerobic fermentation, and ventilate and stir at the same time; the amount of biological treatm...

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Abstract

The invention provides bacillus licheniformis, wherein the classification name is bacillus licheniformis CY-1, the bacillus licheniformis CY-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is the Institute of Microbiology of Chinese Academy of Sciences located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing, the postal code is 100101, the accession number is CGMCC NO.12448, and the preservation date is May 13, 2016. The invention further provides applications of bacillus licheniformis in kitchen waste treatment. The bacillus licheniformis has the activities of high-activity protease, amylase and oil hydrolase, can degrade glucose for generating acid, and is a thermophilic bacterium with high salt tolerance and high acid tolerance, and through effective combination of the bacillus licheniformis with a carrier, the weight of the fed kitchen waste is rapidly and efficiently reduced by 85%.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a licheniformis agent, and also relates to the application of the licheniformis agent in the treatment of kitchen waste. Background technique [0002] Kitchen waste mainly comes from food scraps (kitchen waste) and food residues (swill) from catering operations and residents’ lives. There is a large amount of kitchen waste generated by families, schools, canteens and catering industries in my country. If it is not handled properly, it will pollute the environment, spread diseases, and endanger human health. Food waste has the characteristics of high water content (up to 80-95%), high salinity, high organic matter content, perishable odor and breeding of mosquitoes and flies. The main components are water, sugar, protein, fat, salt and oil, etc. . [0003] At present, the treatment methods of food waste are mainly divided into non-biological treatment (incineration,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14B09B3/00C12R1/10
CPCB09B3/00C12N1/20C12N11/14C12N1/205C12R2001/125Y02W30/78
Inventor 王哲刘立鹤周哲李浩韦祥银张炳如杜国扬
Owner BIOMAX ECOLOGICAL ENG
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