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Biological culture device based on hemodialyzer

A technology for hemodialysis and biological culture, which is applied in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc. It can solve the problems of difficult to achieve dynamic simulation and complicated control of culture conditions.

Active Publication Date: 2016-12-07
NANJING NORMAN BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, the dynamic simulation of drug changes is difficult to achieve, and the control of culture conditions (same as stem cell culture) is cumbersome

Method used

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  • Biological culture device based on hemodialyzer
  • Biological culture device based on hemodialyzer
  • Biological culture device based on hemodialyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0096] Such as Figures 1 to 12 As shown, a biological culture device based on a hemodialyzer includes a hemodialyzer 12, a circulating pump, a swing weighing device, a sample collection and storage device 13, a drug delivery device 5, and a culture bag 8-10, and the culture bag Including a main culture bag 6 and a secondary culture bag 7, the main culture bag 6 is connected to the inner two ends of the main body of the hemodialyzer 12 to form a main circulation loop 1, and the said secondary culture bag 7 is connected to the outside of the main body of the hemodialyzer 12 The two ends are connected to form a secondary circulation loop 2, and the circulation pump includes a main circulation pump 3 and a secondary circulation pump 4, and the main circulation pump 3 is arranged on the pipeline between the main culture bag 6 and the hemodialyzer 12, so The secondary circulation pump 4 is arranged on the pipeline between the secondary culture bag 7 and the hemodialyzer 12, the swi...

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Abstract

The invention provides a biological culture device based on a hemodialyzer. The biological culture device comprises the hemodialyzer, a circulating pump, a swing weighing device, a sample collecting and storing device, a medicine-dosing device and a culture bag. The culture bag comprises a main culture bag and an auxiliary culture bag. The main culture bag is connected with the two ends inside a hemodialyzer main body to form a main circulation loop, and the auxiliary culture bag is connected with the two ends outside the hemodialyzer main body to form an auxiliary circulation loop. The sample collecting and storing device and the medicine-dosing device are connected with a pipe between the main culture bag and the hemodialyzer. The biological culture device is a cell or bacterium culture device which can monitor culture conditions, keep the monitor culture conditions stable, perform automatic liquid adding and sample, can simulate medicine concentration changes and is based on the hemodialyzer.

Description

technical field [0001] The invention relates to the field of biological cultivation, in particular to a biological cultivation device based on a hemodialyzer. Background technique [0002] Stem cell culture refers to the technique of simulating the physiological environment in the body in vitro, cultivating cells removed from the body, and making them survive and grow. In vitro cell culture must pay attention to three links: nutrition, living environment and waste liquid removal. The nutrients required for culturing cells in vitro are provided by the culture medium. In vitro culture must mimic the in vivo cell growth environment. Environmental factors mainly refer to sterile environment, suitable temperature, certain osmotic pressure and gas environment. Stem cells are undifferentiated cells with self-renewal, high proliferation and multiple differentiation potentials. The generation and regulation of various functional cells depend on the interaction and balance of vari...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34A61L2/10
CPCC12M23/14C12M23/58C12M29/02C12M39/00C12M41/00C12M41/12C12M41/26C12M41/34C12M41/40
Inventor 童明庆叶森
Owner NANJING NORMAN BIOLOGICAL TECH
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